• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Specie identification from single amphistome eggs by PCR-RFLP based on ribosomal DNA ITS-2 marker

Research Project

Project/Area Number 10660278
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionIWATE UNIVERSITY

Principal Investigator

ITAGAKI Tadashi  Iwate University, Department of Parasitology, Associate Professor, 農学部, 助教授 (80203074)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsamphistome / Calicophoron calicophorum / Orthocoelium streptocoelium / Homalogaster paloniae / Ribosomal DNA / Nucleotide sequence / ITS-2 / PCR-RFLP / Orthocoelium streptocoelium / Homalogaster paloniae / Calicophoron microbothlium / DNA
Research Abstract

The purpose of this study is first to describe the extent of ITS2 sequence difference among 3 species of amphistomes, namely Calicophoron calicophorum, Orthocoelium streptocoelium and Homalogaster paloniae, and secondly to assess PCR-RFLP method for the species identification of single eggs of the parasites. The length of ITS2 sequence was 284bp in C. calicophoronm and O. streptocoelium, and 285bp in H. paloniae. The ITS2 sequences of the three species contained 19variable sites including one gap and sequence difference between species was 4.2-5.3%. Restriction maps were constructed for ITS2 sequences of the three species. Although several sites existed for a number of common restriction endonucleases, the three species of amphistomes could be distinguished from each other by the position of Acc II, Mfl I and Hha I. These results suggest that ITS2 sequence should be useful as DNA marker for species identification of single eggs of the three species using PCR-RFLP technique.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Itagaki, T., N. Tsumagari , K.Tsutsumi: "Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS-2 marker Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS-2 marker"submitteed to Parasitology Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Itagaki, T., Tsumagari, Y. and Tsutsumi, K: "Species differentiation of paramphistomoidea based on PCR-linked RFLP of ITS-2 of ribosomal DNA"Parasitology International. 47 (suppl). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi