| Project/Area Number |
10660280
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Tokyo University of Agriculture & Technology |
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Principal Investigator |
ARAI Katsuhiko Tokyo University of Agriculture and Technology, Department of Tissue Physiology, Associate Professor, 農学部, 助教授 (60175940)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | MMP / TUMOR / INTEGRIN / SIGNAL TRANSDUCTION / がん / 転移 |
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| Research Abstract |
To investigate the participation of MMP-9 in the formation of lung metastasis, we have established an animal model for lung metastasis by the injection of osteosarcoma (OS) cells into mammary fad pad of SCID mice. Gelatinzymography revealed a great deal of MMP-9 secreted by the lung tissues containing metastatic lesions, whereas only MMP-2 was detected in 'hypothetic' origin these results suggested that OS cells could acquire the ability of MMP-9 expression during tumor invasion and metastasis. Therefore, we examined the mechanism of MMP-9 expression under several culture condition in vitro. In monolayer culture of OS cells on several extracellular matrix, MMP-9 was not detected by gelatin zymography, while MMP-2 was constantly expressed. When OS cells were cultivated in type I collagen gel, MMP-9 was apparently detected in addition of MMP-2. The expression of MMP-9 in collagen gel was down-regulated by anti-integrin antibodies and cytochalasin D. Thus, we suggested that the expression of MMP-9 by OS cells was regulated through integrin-mediated cell-matrix interaction and following actin polymerization.
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