ANALYSIS OF SATELLITE DNA SEQUENCE OF SUNCUSU MURINUS.

• Project/Area Number: 10660281
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
• Principal Investigator: KANDA Naotoshi DEPARTMENT OF VETERINARY MEDICINE TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, 農学部, 教授 (40075429)
• Project Period (FY): 1998 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: CHROMOSOME / CENTROMEA / SUNCUS MURIMUS / SATELLITE DNA / MICROSATELLITE / サテライト DNA / スンクス / FISH

Research Abstract

We have cloned six satellite DNA fragments (pSBs) from the BamHI digested genomic DNA of Suncus murinus. These clones were all mapped to the subcentromeric regions coinsistent with the locations of heterochromatic C-banded regions. Sequence analysis of these clones showed that approximately 3000 copies of the (TTC) repeat were clustered in the satellite DNA.FISH analysis using oligonucleotide (TTC)6 as a probe revealed that (TTC)6 was preferentially hybridized to only one of the two sister chromatids. Such asymmetric hybridization on the sister chromatids has not yet been reported in any probes used in the past. To elucidate the biological significance of the asymmetric hybridization of the (TTC)n repeat, we proposed the "Sister chromatid printing hypothesis" which suggest that each of the sister chromatids might be differentially modified to distinguish chromatids by the unknown genetic mechanisms. This machinary may control the selective segregation of the sister chromatids, resulting in the allocation of a definite set of sister chromatids to the daughter cells.

Research Products

• [Publications] Motomura,S. et al.: "Inhibition of P-glycoprotein and recovery of drug sensitivity of human acute leukemic blast cells"Blood. 91. 3163-3171 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Phipps M. et al.: "A preliminary report on DNA sequence analysis of whale meat and whale meat products collected in Japan"TRAFFIC Bulletin. 17. 91-94 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sakamaki,K. et al.: "The eosinophilic peroxidase gene forms a cluster with the genes for myeloperoxidase and lactoperoxidase on human chromosome 17"Cytogenet. Cell Genet.. 88. 246-248 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kohara,Y. et al.: "A major determinat QTL responsible for atopic dermatis-like skin lesion in NC/Nga mice is located on chromosome 9"Immunogenetics. (in press). (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Motomura,S. et al.: "Inhibition of P-glycoprotein and recovery of drug sensitivity of human acute leukemic blast cells"Blood 91. 91. 3163-3171 (1998)
• [Publications] Phipps M. et al.: "A preliminary report on DNA sequence analysis of whale meat and whale meat products collected in Japan"TRAFFIC Bulletin. 17. 91-94 (1998)
• [Publications] Sakamaki, K. et al.: "The eosinophilic peroxidase gene forms a cluster with the genes for myeloperoxidase and lactoperoxidase on human chromosome 17"Cytogenet. Cell Genet.. 88. 246-248 (2000)
• [Publications] Kohara,Y. et al.: "A major determinat QTL responsible for atopic dermatis-like skin lesion in NC/Nga mice is located on chromosome 9"Immunogenetics. (in press). (2001)
• [Publications] Sakamaki,N. et al.: "The eosinophil peroxidasae gene with the myeloperoxidase and lactoperoxidase genes forms a cluster on human chromosome 17."Cytogenetics Cell Genetics. (in press).
• [Publications] Motomura,S.et al.: "Inhibition of P-glycoprotein and recovery of drug sensitivity of human acute leukemic blast cells" Blood. 91. 3163-3171 (1998)
• [Publications] Phipps M.et al.: "A preliminary report on DNA sequence analysis of whale meat and whale meat products collected in Japan" TRAFFIC Bulletin. 17. 91-94 (1998)