Studies on the application of DNA-based vaccination in the field of veterinary science

• Project/Area Number: 10660294
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Nippon Institute for Biological Science
• Principal Investigator: TUCHIYA Kotaro Nippon Inst. Biol. Sci., Researcher, 研究部, 研究員 (70207405)
• Co-Investigator(Kenkyū-buntansha): KATSUMATA Atsushi Nippon Inst. Biol. Sci., Researcher, 研究部, 研究員 (80270528) ; HOSHI Sumio Nippon Inst. Biol. Sci., Researcher, 研究部, 研究員 (10157000) ; 金井 一享 財団法人日本生物科学研究所, 研究部, 研究員 (50281598)
• Project Period (FY): 1998 – 1999
• Project Status: Completed (Fiscal Year 1999)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: Nucleic acid inoculation / DNA vaccine / Rabies / Canine distemper / Promoter / イヌジステンバー

Research Abstract

DNA-based vaccination is a latest addition to the field of vaccinology, and many basic researches have been done by using experimental animals. This study was conducted to the application of the new technology of vaccination in a combination of dogs and rabies, and the following results were obtained.
1. Of several eukaryotic expression vectors, pCAGGS and pCI plasmid vectors were most effective for the DNA-based vaccination.
2. Dogs received a 100 μg pCAGGS plasmid which were inserted rabies virus glycoprotein gene elicited rabies virus neutralizing antibody. The titers were similar to that elicited by inactivated conventional rabies vaccine. The produced antibody was detected for more than 6 months.
3. About half of dogs which received 10 μg of the plasmid DNA were observed to the antibody response.
These results indicate that DNA-based vaccination against rabies is effective in providing humoral immune response and possibly protective immunity in dogs.

Research Products

• [Publications] K. Tuchiya, I. Sato, Katsumata, A. Iwata, A. Yamamoto, J.Miyazaki, and S. Ueda: "Immunizatio with plasmid DNA encoding rabies virus glycoprotein induces humoral immune response in dogs"Proceedings of International Veterinary Cytokine and Vaccine Conference(I.V.C.V.C). 89-92 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K.Tuchiya, I.Sato, A.Katsumata, A.Iwata, A.Yamamoto, J.Miyazaki, and S.Ueda: "Immunization with a plasmid DNA encoding rabies virus glycoprotein induces humoral immune response in dogs"Proceedings of International Veterinary Cytokine and Vaccine Conference. 89-92 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K.Tsuchiya,I.Sato,A.Katsumata,A.Iwata,A.Yamamoto,J.Miyazaki and S.Ueda: "Immunizatio with a plasmid DNA encoding rabies virus glycoprotein induces humoral immune response in dogs"Proceedings of International Veterinary Cytokine and Vaccine Conference(I.V.C.V.C). 89-92 (2000)