| Project/Area Number |
10660296
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KARIWA Hiroaki Hokkaido Univ., Grad. School of Vet. Med., Asso. Pro., 大学院・獣医学研究科, 助教授 (70224714)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUTANI Tetsuya Hokkaido Univ., Grad. School of Vet. Med., Inst., 大学院・獣医学研究科, 助手 (70281681)
ARIKAWA Jiro Hokkaido Univ., Grad. School of Med., Pro., 医学部, 教授 (10142704)
TAKASHIMA Ikuo Hokkaido Univ., Grad. School of Vet. Med., Pro., 大学院・獣医学研究科, 教授 (30002083)
YOSHIMATSU Kumiko Hokkaido Univ., Grad. School of Med., Inst., 医学部, 助手 (90220722)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Hantavirus / Hemorrhagic fever / Rodent / Emerging infection / Zoonosis |
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| Research Abstract |
Epidemiological study was conducted in Japan and Far East Russia and the followings were revealed.
1. Hantavirus was identified in the urine from infected rats. Since newborn rats intranasally inoculated with the urine containing virus had the virus in their organs and urine, the virus may be maintained by the transmission with virus-contaminated urine by intranasal route in nature.
2. Enzyme linked immunosorbent assay (ELISA) using baculovirus-expressed nucleocapsid proteins of different hantaviruses as antigens was established. This assay made it possible to detect hantaviral antibody and also identify the type of infected hantavirus when sera were reacted with different antigens.
3. To evaluate the virus load in patients of hantavirus pulmonary syndrome (HPS), quantitative PCR system was established. According this PCR, it was revealed that high amount of virus was detectable in blood of acute HPS patients but the virus was disappeared form blood in convalescent phase.
4. Large scale of epidemiological study of hantavirus was conducted in Japan by using severa serological methods. In healthy adults, no one had hantaviral antibody while in hepatic disease patients of unknown etiology, 2 to 3 % of cases had the antibodies. Therefore, it is no doubt that the general public in Japan have hantavirus infections.
5. Epizootiologic study of hantavirus targeting for rodents was carried out in Vladivostok, Far East Russia. Hantaviral antibody was detected in gray-backed vole (Clethrionomys rufocanus), hich is known to be a reservoir of Puumala type hantavirus in Hokkaido, Japan. Since stripped field mice (Apodemus agrarius), which is a reservoir of Hantaan type hantavirus in China and Korea, also had the antibodies, highly virulent strain may be prevalent in Far Eastern Russia. In addition, hantavirus genome was identified in reed vole (Microtis fortis) and sequence analysis revealed that this virus was distinct from other known hantaviruses.
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