| Project/Area Number |
10660297
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
XUAN Xuenan Obihiro University of Agriculture and Veterinary Medicine , Associate Professor, 原虫病研究センター, 助教授 (10292096)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Noboru Obihiro University of Agriculture and Veterinary Medicine , Assistant Professor, 原虫病研究センター, 講師 (10271751)
IGARASHI Ikuo Obihiro University of Agriculture and Veterinary Medicine , Professor, 原虫病研究センター, 教授 (80159582)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | C.parvum / P15 / P23 / Recombinant vaccine / Vaccinia virus / Herpesvirus / 原虫感染防御 / 組換え抗原 / クリプトスポリジウム / ウイルスベクター / P23遺伝子 / P15遺伝子 / ワクチン |
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| Research Abstract |
In host-parasite interaction, the surface proteins of both host and parasite cells play an important role and the surface antigens of the parasites are therefore logical targets for subunit vaccine development. Several surface antigens, such as P15 and P23 of Cryptosporidium parvum (C.parvum) sporozoites or merozoites have been reported previously. Since the monoclonal antibodies against these antigens can neutralize the C.parvum sporozoites, the antigens were proposed as candidates for development of recombinant vaccine to control the cryptosporidiosis. In the present study, we constructed recombinant vaccinia virus or herpesvirus wich expresses the P15 or P23 of C.parvum and demonstrated that the recombinant P15 and P23 are similar to the native P15 and P23 both in structure and antigenicity. Mice or rabbits inoculated with the recombinant viruses produced high titers of antibodies against C.parvum sporozoites. Our next step will be immunization trails with cows to check the potency of the hyperimmune colostrum to confer effective passive immunity of humans and animals against cryptosporidiosis.
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