| Project/Area Number |
10660299
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied veterinary science
|
|---|
| Research Institution | Gifu University Faculty of Agriculture |
|---|
Principal Investigator |
FUKUSHI Hideto Gifu University Dept. Vet. Microbiol., Associate Professor, 農学部, 助教授 (10156763)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA Tomio JRA, Lab. Mol. Biol. Senior Researcher, 競走馬総合研究所・栃木支所・分子生物学研究室, 研究役
YAMAGUCHI Tsuyoshi Gifu University Dept. Vet. Microbiol., Assistant Professor, 農学部, 助手 (70210367)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
|
|---|
| Keywords | herpesvirus / neuropathogenicity / hamster |
|---|
| Research Abstract |
We investigated the pathogenicity of EHV-9 in horses. The horse inoculated with EHV-9 showed pyrexia, verifier and viral excretion in nasal discharge without any neurological symptoms. However, nonsuppurative encephalitis was confirmed. These results indicated that EHV-9 using hamsters. We serially passaged EHV-9 in the rabbit kidney cell line, RK-13. After 23 passages, we observed smaller plaques of EHV-9 in MDBK cells. This small plaque forming EHV-9 culture has less pathogencity in hamsters. We cloned the small plaque forming EHV-9. The cloned viruses also showed less pathogencity in hamsters. These results indicated that the plaque sizes in MDBK cells are related to the pathogenicity in hamsters.
|
