Stimulation of gene transfer efficiency by treatment of human cells with topoisomerase II inhibitors
| Project/Area Number |
10660317
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Yokohama City University |
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Principal Investigator |
ARATANI Yasuaki Kihara Institute for Biological Research, Assistant professor, 木原生物学研究所, 助手 (30192470)
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| Co-Investigator(Kenkyū-buntansha) |
KOYAMA Hideki Kihara Institute for Biological Research, professor, 木原生物学研究所, 教授 (40085626)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | mammalian cell / gene transfer / topoisomerase / bleomycin / nonhomologous recombination / DNA |
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| Research Abstract |
In mammalian cells, nonhomologous recombination is a predominant pathway to repair DNA double-strand breaks. We have shown that DNA topoisomerase II inhibitors are capable of enhancing random integration of foreign DNA via nonhomologous recombination. Since this enhancement is likely due to stabilized DNA strand breaks, we examined the effect of radiomineric antitumor drug, bleomycin, on nonhomologous recombination. We found that bleomycin greatly enhances the random integration of transfected plasmids into human cells. Also, we analyzed the integration junctions between transfected DNA and human chromosomes in clones derived from topoisomerase II inhibitor-treated and-untreated cultures as well as the characterization of inserted DNA found in integration junctions between transfected DNA and human chromosomes. These studies will facilitate the understanding of DNA integration events through nonhomologous recombination and the development of transfection protocols with higher efficiencies.
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Report
(3 results)
Research Products
(15 results)
