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The study of G protein that is associated with ciliary motor function

Research Project

Project/Area Number 10670013
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionMie University

Principal Investigator

SHINOHARA Haruo  Mie University, Faculty of Medicine, associate professor, 医学部, 助教授 (90187378)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Keywordscilia / ventricular cavity / ependymal cells / G protein / rats
Research Abstract

This study was undertaken to clarify the localization of pertussis toxin-sensitive G protein in the cilia of tissues, involving the ependymal cells lining the ventricular cavity of nervous system, the tracheal epithelium, the epithelium of the oviduct.
Adults rats were anesthetized and fixed by perfusion through the heart and issues from brains, trachea, and oviducts were dissected out. Sections for light and electron microscopic examination were processed using antibodies against α subunits of Go and Gi subtypes (Gi1, Gi2, Gi3). In addition, the cilia-rich pellet from the ventricular surface of brains were obtained and the richmess of pertussis toxin-sensitive G protein was shown in this pellet compared with the pellet from the motor cortex.
We found Gi2 was exclusively localized, at light microscopic level, in the cilia of ependymal cells, the cilia of tracheal epithelium and oviduct epithelium. Immunoelectron microscopy revealed that Gi2 was found predominantly in the ciliary membrane of these tissues. Immunoblot analysis demonstrated higher levels of Gi2 in the ependymal cilia-rich pellet than in the motor area of the parietal cortex, supporting the Immunohistochemical observations.
There are some reports that when Bordetella pertussis us inoculated in vivo into the cerebral ventricles of mice, the bacteria multiply only around the ciliated ependymal cells, while the infection by bacteria affected only the ciliated respiratory cells of cultured trachea and caused their ciliostasis with nonciliated cells almost intact. These observations and the results of the present study suggest that Gi2 is localized in the ciliary membrane of ependymal cells and ciliated epithelial cells of trachea and oviduct and seems to be involved in the motile function of these ciliated cells.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Haruo Sinohara: "Localization of aG protein in the cilia of ratependyma, ovidust and trachea"European Journal of Neuroscience. 10. 699-707 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Masami Oguni: "Does light stumulus at eye opening of the developing rat influence retinal expression of GTP-binding protein (Go)?"Ophthalmic Research. 30. 84-89 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Rika Morishita: "High expression of the γγ"Journal of Neurochemistry. 73. 2369-2374 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shinohara H et al.: "Localization of a G protein Gi2 in the cilia of rat ependyma, oviduct and trachea"Eur J Neurosci. 10. 699-707 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oguni M et al.: "Does light stimulus at eye opening of the developing rat influence retinal expression of GTP binding protein (Go) ?"Ophthalmic Res.. 30. 84-89 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morishita R et al.: "High expression of the γ5 isoforms of G protein in neuroepithelial cells and its replacement of the γ2 isoform during neuronal differentiation in the rat brain"J Neurochem. 73. 2369-2374 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Haruo Sinohara: "Localization of a G protein in the cilia of rat ependyma,oviduct and trachea"European Journal of Neuroscience. 10. 699-707 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masami Oguni: "Does light stumulus at eye opening of the developing rat influence retinal expression of GTP-binding protein(Go)?"Ophthalmic Research. 30. 84-89 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Rika Morishita: "High expression of the γ5 isoform of G protein in neuroepithelial cells and its replacement of the γ2 isoform during neuronal differentiation in the rat brain"Journal of Neurochemistry. 73. 2369-2374 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Shinohara: "Localization of a G protein in the cilia of rat ependyma,oviduct and trachea." European Journal of Neuroscience. 10(2). 699-707 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Oguni M,: "Does light stimulus at eye opening of the developing rat in fluence retinal expression of GTP-binding protein (Go)?" Ophthalmic Research. 30. 84-89 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Oguni: "Calbindin-D 28kD and parvalbumin in the horizontal cells of rat retina during development" Current Eye Research. 17. 617-622 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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