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Regulatory role of seronin-containing cells in the gastrointestinal tracts

Research Project

Project/Area Number 10670014
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionShiga University of Medical Science

Principal Investigator

FUJIMIYA Mineko  Shiga Univ. Med. Sci., Dept. Anatomy, Associate Professor, 医学部, 助教授 (10199359)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Hiroshi  Shiga Univ. Med. Sci., Dept. Anatomy, Research Associate, 医学部, 助手 (00283557)
OKUMIYA Kiyohito  Kochi. Med. Coll., Dept. Geriatrics, Research Associate, 医学部, 助手 (20253346)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsserotonin / EC cells / Gastrointestinal Tracts / Perfused intestine / 管腔分泌 / ラット / 十二指腸 / VIP / PACAP
Research Abstract

The mechanism to regulate the release of serotonin (5HT) from the enterochromaffin (EC) cells was investigated in the isolated vascularly and luminary perfused rat duodenum. Results showed that cholinergic nerves stimulated the luminal release of 5HT into the intestinal lumen via muscarinic receptors on the basal site of the EC cells, while VIP-and PACAP-containing nerves inhibited the luminal release of 5HT via VIP type 2 receptor on the basel site of EC cells. VIP receptor exerted the inhibitory effects in the presence of nitric oxide. Immunoelectron microscopic study demonstrated the morphological evidence for the luminal release of 5HT and chromogranin A from the EC cells. The effect of 5HT in the motor activity of the intestine was examined in the perfused intestine. Results showed that 5HT played an important role to keep the perfused intenstine. Furthermore the role of 5HT in the pathogenesis of inflammatory bowel disease was also examined by using the rat model of ulcerative colitis. The density of 5HT-containing EC cells and mast cells as well as the tissue 5HT content was significantly increased especially in the proximal and distal colon. These results suggest that 5HT plays an important role to regulate the gastrointestinal functions.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] M. Fujimiya, H. Yamamoto, A. Kuwahara: "Effect of VIP and PACAP on the basal release of serotonin from isolated vascularly and luminally perfused rat duodenum"Am. J. Physiol.. 275. G731-G739 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Fujimiya, H. Yamamoto, A. Kuwahara: "Effecto of VIP and PACAP on Vascular and luminal release of serotonin from isolated perfused rat duodenum"Ann. NY Acad. Sci.. 865. 495-502 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamamoto, M. Fujimiya Y. Shirai, N. Saito: "Immunohistochemical localization of serotonin transporter in the rat brain"Neurosci. Res.. 32. 305-312 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Fujimiya, K. Okumiya, Y. N. Kwok, C. McIntosh: "Immunoelectron-microscopic study of differential localization of motilin and serotonin in the rabbit duodenal epithelium"Peptides. 19. 65-73 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamamoto, A. Kuwahara, I. Yamamoto, M. Fujimiya: "Motor activity of vascularly perfused rat duodenum. Characteristic of spontaneous movement"Neurogastroenterol. Motil.. 11. 227-234 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamamoto, A. Kuwahara I. Yamamoto, M. Fujimiya: "Motor activity of vascularly perfused rat duodenum Effects of VIP and PACAP27 and PACAP38"Neurogastroenterol. Motil.. 11. 235-241 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Okumiya, M. Fujimiya: "Immunoelectron microscopic study of the luminal release of chromogranin A from rat enterochromaffin cells"Histochem. Cell Biol.. 111. 253-257 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] P-L. Yu, M. Fujimura, K. Okumiya, M. Fuijmiya: "Immunohistochemical localization of tryptophan hydroxylase in the human and rat gastrointestinal tract"J. Comp. Neurol.. 411. 654-665 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Oshima, M. Fujimura, K. Okumiya, M. Fujimiya: "Change in number of serotonin-containing cells and serotonin levels in the intestinal mucosa of rats with colitis induced by dextran sodium sulfate"Histochem. Cell Biol.. 112. 257-263 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] I. Yamamoto, A. Kuwahara, M. Fujimura, M. Fujimiya: "Involvement of 5HT3 and 5HT4 receptors in the motor activity of isolated vascularly perfused rat doudenum"Neurogastroenterol. Motil.. 11. 457-465 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Fujimiya, E. Itoh, N. Kihara, A. Inui: "Neuropeptide Y induces fasted pattern of duodenal motility via Y2 receptors"Am. J. Physiol.. 278. G32-G38 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Fujimiya, H. Yamamoto and A. Kuwahara: "Effect of VIP and PACAP on the basal release of serotonin from isolated vascularly and luminally perfused rat duodenum"American J. Physiol. 275. G731-G739 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Fujimiya, H. Yamamoto and A. Kuwahara: "Effect of VIP and PACAP on vascular and luminal release of serotonin from isolated perfused rat duodenum"Ann. N.Y. Acad. Sci.. 865. 495-502 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamamoto, M. Fujimiya, Y. Shirai, M. Nakashita, M. Oyasu and N. Saito: "Immunohistochemical localization of serotonin transporter in the rat brain"Neurosci. Res.. 32. 305-312 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Fujimiya, K. Okumiya, Y.N. Kwok, S. St-Pierre and C.H.S.McIntosh: "Immunoelectron-microscopic study of differential localization of motilin and serotonin in the rabbit duodenal epithelium"Peptides. 19. 65-73 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamamoto, A. Kuwahara, I. Yamamoto, M. Fujimura, T. Maeda and M. Fujimiya: "Motor activity of vascularly perfused rat duodenum. 1) Characteristic of spontaneous movement"Neurogastroenterol. Mot.. 11. 227-324 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamamoto, A. Kuwahara, M. Fujimura, T. Maeda and M. Fujimiya: "Motor activity of vascularly perfused rat duodenum. 2) Effects of VIP, PACAP27 and PACAP38"Neurogastroenterol. Mot.. 11. 235-241 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Okumiya and M. Fujimiya: "Immunoelectron microscopic study of the luminal release of chromogranin A from rat enterochromaffin cells"Histochem. Cell Biol.. 111. 253-257 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yu P-L., M. Fujimura, K. Okumiya, M. Kinoshita, H. Hasegawa and M. Fujimiya: "Immunohistochemical localization of tryptophan hydroxylase in the human and rat gastrointestinal tract"J. Comp. Neurol. 411. 654-665 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Oshima, M. Fujimura and M. Fujimiya: "Change in number of serotonin-containing cells and serotonin levels in the intestinal mucosa of rats with colitis induced by dextran sodium sulfate"Histochem. Cell Biol.. 112. 257-263 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamamoto, A, Kuwahara, M. Fujimura, M. Kadowaki and M . Fujimiya: "Involvement of 5HT3 and 5HT4 receptors in the motor activity of isolated vascularly perfused rat duodenum"Neurogastroenterol. Mot.. 11. 457-465 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Fujimiya, E. Itoh, N. Kihara, I. Yamamoto, M. Fujimura an A. Inui: "Neuropeptide Y induces fasted pattern of duodenal motility via Y2 receptors in conscious fed rats"Am. J. Physiol.. 278. G32-G38 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Fujimiya,H.Yamamoto,A.Kuwahara: "Effect of VIP and PACAP on the basal release of serotonin from isolated vascularly and luminally perfused rat duodenum"Am. J. Physiol.. 275. G731-G739 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Fujimiya,H.Yamamoto,A.Kuwahara: "Effect of VIP and PACAP on vascular and luminal release of serotonin from isolated perfused rat duodenum"Ann. NY Acad. Sci.. 865. 495-502 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Yamamoto,M.Fujimiya,Y.Shirai,N.Saito: "Immunohistochemical localization of serotonin transporter in the rat brain"Neurosci. Res.. 32. 305-312 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Fujimiya,K.Okumiya,Y.N.Kwok,C.McIntosh: "Immunoelectron-microscopic study of differential localization of motilin and serotonin in the rabbit duodenal epithelium"Peptides. 19. 65-73 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Yamamoto,A.Kuwahara,I.Yamamoto,M.Fujimiya: "Motor activity of vascularly perfused rat duodenum 1) Charateristic of spontaneous movement"Neurogastroenterol. Mot.. 11. 227-234 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Yamamoto,A.Kuwahara,I.Yamamoto,M.Fujimiya: "Motor activity of vascularly perfused rat duodenum 2) Effect of VIP and PACAP27 PACAP38"Neurogastroenterol. Mot.. 11. 235-241 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Okumiya,M.Fujimiya: "Immunoelectron microscopic study of the luminal release of chromogranin A from rat enterochromaffin cells"Histochem. Cell Biol.. 111. 253-257 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] P-L.Yu,M.Fujimura,K.Okumiya,M.Fujimiya: "Immunohistochemical localization of tryptophan hydroxylase in the human and rat gastrointestinal tract"J. Comp. Neurol.. 411. 654-665 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Oshima,M.Fujimura,M.Fujimiya: "Change in number of serotonin-containing cells and serotonin levels in the intestinal mucosa of rats with colitis induced by dextran sodium sulfate"Histochem. Cell Biol.. 112. 257-263 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] I.Yamamoto,A.Kuwahara,M.Fujimura,M.Fujimiya: "Involvement of 5HT3 and 5HT4 receptors in the motor activity of isolated vascularly perfused rat duodenum"Neurogastroenterol. Motil.. 11. 457-465 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Fujimiya,E.Itoh,N.Kihara,A.Inui: "Neuropeptide Y induces fasted pattern of duodenal motility via Y2 receptors"Am. J. Physiol.. 278. G32-G38 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Fujimiya,H.Yamamoto,A.Kuwahara: "Effect of VIP and PACAP on the basal release of serotonin from isolaced Vascularly and luminally perfrsed rat duoderum" American Journal of Physiolgy. 275. G731-G739 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Fujimiya,H.Yamamoto,A.Kuwahara: "Effect of VIP and PACAP on vascular and luminal release of serotonin from isolated perfused rat duoderum" Annals of the New York Academy of Science. 865. 495-502 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Yamamoto,M.Fujimiya,Y.Shirai,N.Saito: "Immunohistochemical localization of seritonin transporter in the rat brain" Neuroscience Research. 32. 305-312 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Yamamoto,T.Maeda,M.Fujimura,M.Fujimiya: "Urocortin-like immunoreactivity in the substantia nigra,ventral tegmental area and Edinger-Westphel nucleus of rat" Neuroscience Letters. 243. 21-24 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Fujimiya,K.Okumiya,YN.Kwok,S.SI-Pierce,C.H.S.MaIntosh: "Immuno-electron microscopic study of differential localization of Motilin and Secotonin in the rabbit duodenel epithe" Peptides. 19. 65-73 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.Funahashi,M.Fujimiya,H.Kimura,T.Maeda: "Abnormal development of serptonin nerve fibers in the visual cortex in rats with methyloxymethanol-induced microcephely" Acta Neuropathologica. 95. 5-14 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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