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Study on the expression of matrix-degrading enzymes and programmed cell death during mammalian development

Research Project

Project/Area Number 10670015
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TAKIGAWA Toshiya  Kyoto University, Department of Anatomy and Developmental Biology, Assistant, 医学研究科, 助手 (90263095)

Co-Investigator(Kenkyū-buntansha) SHIOTA Kohei  Kyoto University, Department of Anatomy and Developmental Biology, Professor, 医学研究科, 教授 (80109529)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsmammal / morphogenesis / programmed cell death / apoptosis / palatogenesis / matrix metalloproteinase / limb development / organ culture / マトリツクスメタロプロテナーゼ
Research Abstract

To elucidate the significance of the PCD and machinery of the morphogenesis of palate and limb, we investigated the occurrence of the PCD and the expression of matrix-degrading enzymes in vivo using mouse fetuses and carried out a series of experiments in vitro. The results obtained are summarized as follows;
1. Palatogenesis
1) PCD in the midline epithelial seam is not always necessary for the mesenchymal confluence of palate shelves, 'fusion'. 2) MEE cells have an ability to autonomously differentiate into mesenchymal cells without a contact or adhesion of the opposing palatal shelves. 3) When MEE cells have fail to transform into mesenchymal cells, the MEE cells appear to undergo apoptotic cell death. 4) Matrix metalloproteinases seem to be a key molecule for not only the mesenchymal confluence, but also the terminal differentiation of MEE cells.
2. Digit separation of limb bud.
1) When PCD occurs in the interdigital tissues prior to digit separation, some of endothelial cells are undergo apoptosis in the regressing marginal venous sinus and interdigital microvascular plexus. 2) PCD in the interdigital tissues appears to directly contribute to the vascular remodeling, but not to digit separation. 3) The expression of gelatinize/MMP-2 mRNA is closely associated with the vascular remodeling that appears to be required for the complete separation of individual digits. 4) In hindlimbs of the Hammer-toe(Hm/Hm) mouse fetuses, the insufficient cell death between digits II and III and between digits III and IV might result in the failure not only of the separation of digits but also of regression of the interdigital microvascular plexus.
In conclusion, during mammalian development, the formation of complicated structures are achieved by 'Scrap and Build' of preexisting structures resulting from the expression of matrix-degrading enzymes and the occurrence of PCD.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Takigawa,T. and Shiota,K.: "Significance of programmed cell death /apoptosis during mammalian development with special reference to palate and limb morphogenesis"Acta Histochemica et Cytochemica. 32(6). 510 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takigawa,T., et al.: "Study of the vascular remodelling and programmed cell death during the separation of digits in the developing mouse limb"Acta Anatomica Nipponica. 75(1). 67 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takigawa,T. and Shiota,K.: "Study of the differentiation of the medial edge epithelium in the developing mouse fetal palate by using a suspension single shelf culture"Acta Anatomica Nipponica. 75(1). 60 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 滝川俊也、塩田浩平(分担): "臓器別アポトーシス証明法 大槻勝紀、小路武彦、渡辺慶一(編集) 第III章 C.消化器系 1.口腔とアポトーシス pp.116-117"南江堂. 259 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takigawa, T., et al.: "Study of the vascular remodelling and programmed cell death during the separation of digits in the developing mouse limb"Acta Anatomica Nipponica. 75 (1). 67 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takigawa, T. and Shiota, K.: "Study of the differentiation of the medial edge epithelium in the developing mouse fetal palate by using a suspension single shelf culture"Acta Anatomica Nipponica. 75 (1). 60 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takigawa, T. and Shiota, K.: "Significance of programmed cell death/apoptosis during mammalian development with special reference to palate and limb morphogenesis"Acta Histochemica et Cytochemica. 32 (6). 510 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takigawa,T.and Shiota,K.: "Significance of programmed cell death/apoptosis during mammalian development with special reference to palate and limb morphogenesis"Acta Histochemica et Cytochemica. 32(6). 510 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takigawa,T.et al.: "Study of the vascular remodelling and programmed cell death during the separation of digits in the developing mouse limb"Acta Anatomica Nipponica. 75(1). 67 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takigawa,T.and Shiota,K.: "Study of the differentiation of the medial edge epithelium in the developing mouse fetal palate by using a suspension single shelf culture"Acta Anatomica Nipponica. 75(1). 60 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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