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ATP release in neuroepithelium of early embryonic retina

Research Project

Project/Area Number 10670042
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionNara Medical University (1999)
Osaka University (1998)

Principal Investigator

YAMASHITA Masayuki  Nara Med.Univ., Faculty of Med., Prof., 医学部, 教授 (20183121)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordsretina / development / adenosine triphosphate / neuroepithelium / chick embryo / organ culture / proliferation / calcium ion / アデノシン3リン酸
Research Abstract

Adenosine triphosphate (ATP) activates P2 purinoceptors. The activation of P2 purinoceptors induces CaィイD12+ィエD1 mobilization (release of CaィイD12+ィエD1from intracellular CaィイD12+ィエD1 stores) in the early embryonic chick neural retina during the period when the retinal cells have mitotic activities. After the CaィイD12+ィエD1 mobilization the entry of extracellular CaィイD12+ィエD1 (capacitative CaィイD12+ィエD1 entry) also occurs in the embryonic retina. To investigate the role of P2 purinoceptors and CaィイD12+ィエD1 mobilization in the regulation of retinal cell proliferation, the effects of the P2 purinoceptor antagonists and of the against ATP on DNA synthesis were studied in retinal organ cultures from embryonic day 3 (E3) chick. The antagonists inhibited [ィイD13ィエD1H]-thymidine incorporation in a dose-dependent manner and ATP enhanced [ィイD13ィエD1H]-thymidine incorporation to maximally 200% of control. The release of ATP was demonstrated in the medium of retinal organ cultures. The concentration of ATP increased 25-fold within one hour of incubation and this concentration was kept for at least 24 hours. These results indicated that P2 purinoceptors activated by autocrine or paracrine release of ATP were involved in the regulation of DNA synthesis in the neural retina at early embryonic stages. Then we studied the effects of an inhibitor of the CaィイD12+ィエD1 pump of intracelluar CaィイD12+ィエD1 stores and an inhibitor of capacitative CaィイD12+ィエD1 entry on the DNA synthesis in the retinal organ cultures from E3 chicks and in dissociated cultures from E7 and E9 chick retinae. It was demonstrated that both antagonists inhibited [ィイD13ィエD1H]-thymidine incorporation in a dose-dependent manner without affecting cell viability or morphology. These results suggested the involvement of CaィイD12+ィエD1 mobilization and capacitative CaィイD12+ィエD1 entry in the regulation of DNA synthesis in the developing neural retina.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Yamashita, M. et al.: "Calcium mobilization systems during neurogenesis"News Physiol. Sci.. 13. 75-79 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山下勝幸: "ATP受容体"Clinical Neuroscience. 16. 1331 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugioka, M. et al.: "Involovement of P2 purinoceptors in the regulation of DNA synthesis in the neural retina of chick embryo"Int. J. Dev. Neurosci.. 17. 135-144 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugioka, M. et al.: "Ca^<2+>mobilization and capacitative Ca^<2+>entry regulate DNA synthesis in cultured chick retinal neuroepithelal cells"Int. J. Dev. Neruosci.. 17. 163-172 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhou, W. -L. et al.: "Regulation of capacitative Ca^<2+>entry by tyrosine phosphorylation in the neural retina of chick embryo"Neurosci. Lett.. 272. 123-126 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] "Lysophosphatidic acid-induced Ca^<2+>mobilization in the neural retina of chik embryo"J. Neurobiol.. 41. 495-504 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamashita M., et al.: "Calcium mobilization systems during neurogenesis."News Physiol. Sci. 13. 75-79 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugioka M., et al.: "Involvement P2 purinoceptors in the regulation of DNA synthesis in the neural retina of chick embryo."Int. J. Dev. Neurosci. 17. 135-144 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugioka M., et al.: "CaィイD12+ィエD1 mobilization and capacitative CaィイD12+ィエD1 entry regulate DNA synthesis in cultured chick retinal neuroepithelial cells."Int. J. Dev. Neurosci. 17. 163-172 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhou W.-L., et al.: "Regulation of capacitative CaィイD12+ィエD1 entry by tyrosine phosphorylation in the neural retina of chick embryo."Neurosci. Lett. 272. 123-126 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhou W.-L., et al.: "Lysophosphatidic acid-induced CaィイD12+ィエD1 mobilization in the neural retina of chick embryo."J. Neurobiol. 41. 495-504 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morigiwa,K.et al.: "P2 purinoceptor expression and functional changes of hypoxia-activated cultured rat retinal microglia"Neurosci.Lett.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zhou,W.-L.et al.: "Regulation of capacitative Ca^<2+> entry by tyrosine phosphorylation in the neural retina of chick embryo"Neurosci.Lett.. 272. 123-126 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zhou,W.-L.et al.: "Lysophosphatidic acid-induced Ca^<2+> mobilization in the neural retina of chick embryo"J.Neurobiol.. 41. 495-504 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugioka,M.et al.: "Ca^<2+> mobilization and capacitative Ca^<2+> entry regulate DNA synthesis in cultured chick retinal neuroepithelial cells"Int.J.Dev.Neurosci.. 17. 163-172 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugioka,M.et al.: "Involvement of P2 purinoceptors in the regulation of DNA synthesis in the neural retina of chick embryo"Int.J.Dev.Neurosci.. 17. 135-144 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miho Sugioka,et al.: "Involvement of P2 purinoceptors in the regulation of DNA synthesis in the neural retina of chick embryo." Int.J.Dev.Neurosci.(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masayuki Yamashita,et al.: "Calcium mobilization systems during neurogenesis." News Physiol.Sci.13. 75-79 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山下勝幸,杉岡美保: "神経発生期でのATP受容体によるCa動員" 岡崎国立共同研究機構生理学研究所年報. 19. 299- (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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