Water in Sciatic Nerve Measured by Double-Quantum-Filtered NMR

• Project/Area Number: 10670046
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: SEO Yoshiteru Dept.of Physiology, Lecturer, 医学部, 講師 (90179317)
• Project Period (FY): 1998 – 1999
• Project Status: Completed (Fiscal Year 1999)
• Budget Amount: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: Water / Quadrupolar interaction / Diffusion / Multiple-quantum NMR / PFG NMR / Degeneration / Intracellular compartmentation / 末梢神経 / 座骨神経 / パルス磁場勾配NMR法

Research Abstract

The Aims of this project is 1) to reach an understanding of factors governing the NMR relaxation of water in peripheral nerve tissue and 2) to separate water compartmentation based on parameters of H-2 double-quantum relaxation to follow "order" structure of water and to use it to understand physiological processed in healthy and pathological tissues.
1) Compartmentation of water in sciatic nerve of rats : Three compartments of ィイD12ィエD1HィイD22ィエD2O in anisotropic motion condition were detected by H-2 DQF NMR. The observed residual quadrupole splitting were ca. 480, 125 and 9 Hz at 24℃ which were assigned as ィイD12ィエD1HィイD22ィエD2O in endoneurium, epineurium, and axons respectively. The three DQF signals showed anisotropic diffusion, but only the axoplasm compartment represented restricted diffusion. From restricted diffusion of the axoplasm water, the inner-diameter of axon was estimated as 7.5 um.
2) Development of flow-cell type NMR probe : We have made a flow-cell type of H-2 NMR probe. Using a glass capillary (1.5 mm inner diameter), perfusate of the sciatic could be switched within 10 sec which allows us to measure uni-directional water exchange between tissue compartments.
3) Assessment of degeneration of sciatic nerve : Changes in water exchange and H-2 DQF relaxation parameters were measured on the Wallerian degeneration nerve. Water permeability increased and water structures in the 3 compartments degenerated by 1-2 weeks. It is possible to follow the degeneration process by H-2 DQF NMR.

Research Products

• [Publications] Sharf Y. et al.: "Mapping strain exerted on blood vessel walls using deuterium double-quantum-filtered MRI"Proc. Natl. Acad. Soc. USA. 95. 4108-4112 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Seo, Y. et al.: "Anisotropic and restricted diffusion of water in sciatic nerve : A^2Hdouble-quantum-filtered NMR study"Magn. Reso. Med.. 42. 461-466 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sharf,Y., Seo,Y., Eliav,U., Akselrod,S. & Navon,G.: "Mapping strain exerted on blood vessel walls using deuterium double-quantum-filtered MRI."Proc.Natl.Acad.Soc.USA. 95. 4108-4112 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Seo,Y., Shinar,H., Morita,Y. & Navon,G.: "Anisotropic and restricted diffusion of water in sciatic nerve : A ィイD12ィエD1H double-quantum-filtered NMR study"Magn.Reso.Med.. 42. 461-466 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Seo,Y., Takamiya,H., Ishikawa,H., Nakashima,T., Sharf,Y. & Navon,G.: "Spatially Resolved Magnetic Resonance"P.Bluemler, B.Bluemich, R.Botto, E.Fukushima edited, Wiley-VCH NMR imaging of rigid biological tissues. 445-457 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shinar,H., Sharf,Y., Eliav,U., Seo,Y., & Navon,G.: "Spatially Resolved Magnetic Resonance"P.Bluemler, B.Bluemich, R.Botto, E.Fukushima edited, Wiley-VCH ィイD12ィエD1H double quantum filtered NMR histology and diffusion measurements in isolated nerves and blood vessels. 307-321 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sharf,T.,Seo,Y.,Eliav,U.,Akselrod,S. & Navon,G.: "Mapping strain exerted on blood vessel walls using deuterium double-quantum-filtered MRI"Proc.Natl.Acad.Soc.USA. 95. 4108-4112 (1998)
• [Publications] Seo,Y.,Shinar,H.,Morita,Y. & Navon,G: "Anisotropic and restricted diffusion of water in sciatic nerve : A ^2H double-quantum-filtered NMR study"Magn.Reso.Med.. 42. 461-466 (1999)
• [Publications] Y.Seo: "Anisotropic and restricted diffusion of water in sciatic nerve : A ^2H double quantum filtered NMR study." Magn.Reso.Med.(in press).
• [Publications] Y.Sharf: "Mapping strain on blood vessel walls using deuterium double-quantum-filtered MRI." Proc.Natl.Acad.Soc.USA. 95. 4108-4112 (1998)
• [Publications] H.Shinar: "^2H double quantum filtered NMR histology and diffusion measurements in isolated nerves and blood vessels.in Spatially Resolved Magnetic Resonance" Wiley-VCH, p.307-321 (1998)