| Project/Area Number |
10670052
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | OKAZAKI NATIONAL RESEARCH INSTITUTES |
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Principal Investigator |
MURAKAMI Masataka National Institute for Physiological Science, Department of Molecular Physiology, Associate Professor, 生理学研究所, 助教授 (10104275)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | transport of water and electrolytes / protein secretion / energy supply / oxygen consumption / carbachol / isoproterenol / amylase / parotid gland |
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| Research Abstract |
Energy supply for secretion of fluid and protein was measured as oxygen consumption in the isolated perfuse salivary glands from rats. The parotid glands and submandibular glands from male Wistar strain rats were surgically isolated under anesthesia The artery, vein and secretary duct were canulated and arterially perfused: constant perfusion rate using peristaltic pump. The fluid secretion and amylase (parotid gland), mucin (as N-acetylgalactosamine in submandibular gland) in saliva and oxygen consumption were measured during stimulation with carbachol (CCh) and/or isoproterenol (Isop). Isop alnoe did not induce fluid secretion in paroti gland and the secreted amylase was not measured. CCh alone induced a rapid increase of fluid secretion in botl glands and allowed to measure amylase and mucin. CCh alone induce a initial transient increase of amylase and mucin secretion at 1 min with following decline to a low plateau level. At 5 min from start of CCh stimulation, Isop was added to the perfusion. Fluid secretion and oxygen consumption were not influenced in the submandibular glands, but that of parotid gland was a little increased with additional increment of oxygen consumption. The present study indicates that the increased energy supply in response to secretary stimulation could satisfy the energy requirement mainly for transport of electrolytes and water. On the other hand, the ener requirement for protein secretion in response to secretary stimulation could be relatively small, or under the lim of measurement in the mandibular gland. Responses of mucin and amylase secretion to Ca-mobilizing agent (CCh) and/or cAMP-mobilizing agaent (Isop) were similar in the submandibular and parotid glands, suggesting common machinery of exocytosis for mucin and amylase secretion.
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