| Project/Area Number |
10670159
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | SHIMANE MEDICAL UNIVERSITY |
|---|
Principal Investigator |
MORIKAWA Shigeru SHIMANE MEDICAL UNIVERSITY, PATHOLOGY 1ィイD1STィエD1 UNIT, PROFESSOR, 医学部, 教授 (80027094)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | HODGKIN'S CELL / CELL LINE / CELL ORIGIN / IMMUNOGLOBULIN / B-LINEAGE DENDRITIC CELL |
|---|
| Research Abstract |
Hodgkin's disease is a unique malignant lymphoma characterized by proliferation of Reed-Sternberg (R-S) cells and Hodgkin (H) cells, whose normal counterpart have not yet been identified, although the most recent evidence suggests a lymphoid origin.
This project aim to elucidate normal counterpart of R-S and H cells and further to analysis their biological properties to understand pathogenesis of Hodgkin's disease.
More than 21 long-term cultured cell lines derived from patients with malignant lymphoma and leukemia were investigated their immunological properties> Among them, HPL-Hod-2 (Hod-2), a lymphoblastoid cell line had been established from pleural effusion of a patient with Hodgkin's disease> Hod-2 cells shared common cell markers of R-S and H cells such as, CD15, CD25, CD30 and possessed pretty amount of IgG in their cytoplasms.
But, they lacked surface immunoglobulin expression.
Molecular analysis revealed these IgG consisted of H-chains and k-chains with almost equal molecular size to cytoplasmic IgG of ordinary B-cells.
Interestingly enough, it was found that Hod-2 cells express CD83 on their cell surface. CD83 is thought to be the most reliable makers of dendritic cells.
Among 21 human malignant lymphoma / leukemia cell lines, one hairly leukemia cell one and one B-cell malignant lymphoma cell line express weakly CD83 in their cytoplasms besides Hod-2 cells.
In conclusion ; this investigation revealed the presence of B-lineage DC and their possible original cells of H and R-S cells.
|
