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Genetic study of gastric lymphoma : somatic mutation analysis of VH genes for lymphomagenesis and blastic transformation

Research Project

Project/Area Number 10670170
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionFukushima Medical University School of Medicine

Principal Investigator

HOJO Hiroshi  Fukushima Medical University School of Medicine, Assistant Professor, 医学部, 助教授 (90209213)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Naoya  Fukushima Medical University School of Medicine, Assistant Lecture, 医学部, 講師 (50227922)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsGastric lymphoma / immunohistochemistry / VH genes / Somatic hypermutation / PCR / ongoing mutation / びまん性大細胞型Bリンパ腫 / polymerase chain reaction (PCR)
Research Abstract

To clarify the cell characteristics and origins of low-grade MALT-type lymphoma (L-MALT) and high-grade lesion (H-L), and to investigate the clonal relationships among these tumors, we compared histologic, immunohistologic, and genetic characteristics of L-MALT, H-L, and diffuse large B-cell lymphoma (DLBCL). Our analysis revealed the following results.
1) From data obtained by somatic mutation analysis, L-MALT is thought to originate from post-germinal center B-cells that have undergone antigen selection. The ongoing mutation of L-MALT may reflect reentry into a germinal center pathway.
2) Extranodal DLBCL has a higher somatic mutation frequency, exhibiting antigen-driven high affinity, than that of nodal DLBCL.
3) Histologic and immunohistologic differences were found in L-MALT, H-L, and DLBCL.
4) The frequently mutated VH genes of H-L and DLBCL suggest that these lymphomas may be driven from germinal center or post-germinal center B-cells.
5) The PCR analysis of CDR3 and VH regions of L-MALT and H-L in a successful case revealed different nucleic acid sequences, suggesting that L-MALT and H-L may represent unrelated clones.
Further investigation using single-cell PCR will determine any clonal link and prognostic significance between H-L and DLBCL arising from mucosa-associated lymphoid tissues.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Hiroshi Hojo: "Somatic mutation of immunoglobulin heavy chain variable region genes in gastric low-grade MALT type lymphoma."Med J Kagoshima Univ. 51(supple). 48-50 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naoya Nakamura: "Analysis of the immunoglobulin heavy chain gene variable region of 101 cases with peripheral B cell neoplasms and B cell chronic lymphocytic leukemia in japanese population."Pathology International. 49. 595-600 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiroshi Hojo: "Somatic mutation in immunoglobulin heavy chain variable region genes in gastric low-grade MALT type lymphoma"Med J Kagoshima Univ. 51(suppl). 48-50 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hiroshi Hojo: "Analysis of the immunoglobulin heavy chain gene variable region of 101 cases with peripheral B cell neoplasms and B cell chronic lymphocytic leukemia in the Japanese population."Pathology International. 49. 595-600 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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