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Identification of new tumor antigens using testis-specific proteins

Research Project

Project/Area Number 10670175
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionKeio University

Principal Investigator

FUJITA Tomonobu  Keio University, School of Medicine, Instructor, 医学部, 助手 (20199334)

Co-Investigator(Kenkyū-buntansha) HATA Junichi  Keio University, School of Medicine, Professor, 医学部, 教授 (90051614)
IKEDA Hideyuki  Keio University, School of Medicine, Assistant Professor, 医学部, 専任講師 (40301494)
KAWAKAMI Yutaka  Keio University, School of Medicine, Professor, 医学部, 教授 (50161287)
MURAI Masaru  Keio University, School of Medicine, Professor, 医学部, 教授 (90101956)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsCT antigen / SAGE / SEREX
Research Abstract

Cancer-Testis (CT) antigens that are expressed in normal testis and various tumors are promising targets for immunotherapy for cancer. To identify new CT antigens, we used 2 strategies: Isolation of testis specific genes that also express in tumor cells, and screening of a testis cDNA library with sera from cancer patients. Profile of mRNA of testis was analyzed using SAGE (serial analysis of gene expression), and approximately 20,000 tags containing 12,400 tags for distinct proteins were identified in the testis SAGE library. By comparing it with SAGE databases from selected other normal and cancer tissues, we have obtained candidate tags for new CT antigens. However, more detained analysis of these selected tags revealed that genes containing these tags with high expression were not specific for testis and cancer. Thus, new CT was not identified among these selected genes. Further analysis of selected tags with relatively low expression remains to be performed. The testis cDNA library was screened with sera from 2 pancreatic cancer patients and a bladder cancer patient, and 5 positive clones were obtained. CT-1 isolated with the pancreatic cancer patient's serum was a novel gene not registered in the gene database. IgG antibodies for CT-1 was detected in 1 of 5 sera from pancreatic cancer patients and 1 of 10 sera with renal cancer patients, but was not detected in 20 sera from healthy individuals. CT-1 was expressed in only testis among normal tissues tested and in 1 of 15 pancreatic cancer cell lines and 2 of 5 pancreatic cancer tissues. These results demonstrated that CT-1 was a new CT antigen expressed in some pancreatic cancers.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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