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Expression and LOI of Igf2 in mouse tumors

Research Project

Project/Area Number 10670191
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionASAHIKAWA MEDICAL COLLEGE

Principal Investigator

OGAWA Katsuhiro  Asahikawa Medical College, Department of Pathology, Professor, 医学部, 教授 (50045514)

Co-Investigator(Kenkyū-buntansha) OBATA Masahiko  Asahikawa Medical College, Department of Pathology, Assistant, 医学部, 助手 (70301992)
YOSHIE Masumi  Asahikawa Medical College, Department of Pathology, Assistant, 医学部, 助手 (70292125)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsIgf2 / imprinting / LOI / HCC cells / H19 / methylation / DMR / mice / 肝上皮細胞 / 転写開始点 / RT-PCR
Research Abstract

Igf2 is expressed in most tissues during a fetal period in mice, while it is suppressed except for the brain in adult. Igf2 is an imprinted gene of which paternal allele is expressed, while its material allele is suppressed. It is suggested that the imprinting of Igf2 is maintained by methylation of the specific regions within the Igf2 gene as well as methylation of the H19 gene which is located downstream of Igf2. The Igf2 gene contains 6 exons, and lgf2 mRNA includes one of exons 1-3 with exons 4-6 in common. On the other hand, since most mouse tumors express Igf2 and since tumors occasionally develop in Igf2 transgenic mice, Igf2 is thought to have a role in tumor development. A purpose of this study was to investigate the allele specific Igf2 expression, methylation of Igf2 gene and transcription start points in mouse tumor cells.
Following points became clear in 1998- 1999.
(1) By analyzing the polymorphic CA repeat within Igf2 exon 6, loss of imprinting (LOI) was frequently detected in mouse HCC cell lines. On the other hand, LOI was hardly found in liver epithehal (LE) cells derived from the normal liver.
(2) All the P1, P2, P3 promoters were active in the HCC cells, while only P2 and P3 promoters were active in LE cells.
(3) Igf2 was expressed in the primary cultures of normal hepatocytes, but its imprinting is maintained in this case.
(4) Paternal allele specific methylation of CpG was identified by methylation specific PCR-DNA sequencing in the upstream non-coding region in Igf2.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Ktsuhiro Ogawa: "Loss of imprinting of the insulin-like growth factor II gene in mouse hepatocellular carcinoma cell lines"Mol.Carcinog.. 22. 248-253 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Masumi Yoshie,Katsuhiro Ogawa: "dominant trait in hepatocarcinogenesis-susceptible and resistant mouse strains"Carcinogenesis. 19. 1103-1107 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ktsuhiro Ogawa: "β-catenin mutations are frequent in hepatocellular carcinomas but absent in adenomas induced by diethylnitrosamine in B6C3F1 mice"Cancer Res.. 59. 1830-1833 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ktsuhiro Ogawa: "hepatocellular carcinoma cell lines and primary tumors, but loss of chromosomes 4 and 12 is detected only in the cell lines"Carcinogenesis. 20. 99-108 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ktsuhiro Ogawa: "Expression of an IL-1 receptor antagonist during mouse hepatocarcinogenesis demonstrated by differential display analysis"Lab.Invest.. 79. 1059-1067 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ktsuhiro Ogawa: "in liver epithelial and hepatocellular carcinoma cell lines from B6C3F1 and C3B6F1 mice. Int.J.Cancer(in press)"Int.J.Cancer. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takao Ooasa, Hidenori Karasaki. Hiroaki Kanda, Kimie Nomura, Tomoyuki Kitagawa and Katsuhiro Ogawa: "Loss of imprinting of the insulin-like growth factor II gene in mouse hepatocellular carcinoma cell lines."Mol. Carcinog.. 22. 248-253 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Masumi Yoshie, Hiroyuki Nishimori, Gang-Hong Lee and Katsuhiro Ogawa: "High colony forming capacity of primary cultured hepatocytes as a dominant trait in hepatocarcinogenesis-susceptible and resistant mouse strains."Carcinogenesis. 19. 1103-1107 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katsuhiro Ogawa, Yoshihisa Yamada, Kan Kishibe, Kenichi Ishizaki and Yoshihiko Tokusashi: "Β-catenin mutations are frequent in hepatocellular carcinomas but absent in adenomas induced by diethylnitrosamine in B6C3F1 mice."Cancer Res.. 59. 1830-1833 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katsuhiro Ogawa, Makoto Osanai, Masahiko Obata, Kenichi Ishizaki and Kenji Kamiya: "Gain of chromosomes 15 and 19 is frequent in both mouse hepatocellular carcinoma cell lines and primary tumors, but loss of chromosomes 4 and 12 is detected only in the cell lines."Carcinogenesis. 20. 99-108 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshihisa Yamada, Hidenori Karasaki, Kouji Matsusbima, Gang-Hong Lee and Katsuhiro Ogawa: "Expression of an IL-1 receptor antagonist during mouse hepatocarcinogenesis demonstrated by differential display analysis."Lab. Invest.. 79. 1059-1067 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kenichi Ishizaki and Katsuhiro Ogawa: "Fine mapping of smallest common regions of deletion on chromosome 12 in liver epithelial and hepatocellular carcinoma cell lines from B6C3F1 and C3B6F1 mice."Int. J. Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katsuhiro Ogawa: "Loss of imprinting of the insulin-like growth factor II gene in mouse hepatocellular carcinoma cell lines"Mol. Carcinog.. 22. 248-253 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masumi Yoshie,Katsuhiro Ogawa: "dominant trait in hepatocarcinogenesis-susceptible and resistant mouse strains"Carcinogenesis. 19. 1103-1107 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuhiro Ogawa: "β-catenin mutations are fequent in hepatocellular carcinomas but absent in adenomas induced by diethylnitrosamin in B6C3F1 mice"Cancer Res.. 59. 1830-1833 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuhiro Ogawa: "hepatocellular carcinoma cell lines and primary tumors, but loss of chromosomes 4 and 12 is detected only in the cell lines"Carcinogenesis. 20. 99-108 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuhiro Ogawa: "Expression of an IL-1 receptor antagonist during mouse hepatocarcinogenesis demonstrated by differential display analysis"Lab. Invest.. 79. 1059-1067 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuhiro Ogawa: "in liver epithelial and hepatocellular carcinoma cell lines from B6C3F1 and C3B6F1 mice"Int. J. Cancer. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Ogawa,K.: "Loss of imprinting the insulin-like growth factor II gene in mouse hepatocellular carcinoma cell lines." Mol.Carcinog.23. 248-253 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ogawa,K.: "β-catenin mutations are frequent in hepatocellular curcinomas but absent in adenomas induced by diethylnitrosamine in B6C 3Fl mice" Cancer Res.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshie.M.: "High colony forming capacity of primary cultured hepatocytes as a dominannt trait in hepatocarcinogenesis-susceptible and resistant mouse strains." 19. Carcinogenesis. 1103-1107 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ogawa,K.: "Prolouged function of hepatocytes transplanted into spleens of Nagase analbaminemic rats." Europ.J.Surg.(in press).

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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