| Project/Area Number |
10670192
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATOH Hitoshi The Institute of Medical Science, Department of Pathology, The University of Tokyo, Research Associate, 医科学研究所, 助手 (70183829)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | 1p36 translocation / malignant lymphoma / FISH / cosmid / YAC |
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| Research Abstract |
We have arrayed 21 cosmid and five P1 phage clones along with the short arm of chromosome 1 from telomere to the centromere direction by pair-wise comparison experiment using multi-color FISH technique. The resulting order was as follows : 1pter-D1S1002 (cYS142)-D1S1053 (cYS1467)-D1Z2-D1S1085 (cYS1138)-D1S1013 (cYS1299)-D1S1032 (cYS1384)-D1S1010 (cYS1296)/D1S1047 (cYS1429)-D1S96-D1S989 (cYS1287)/D1S1131 (cYS1234)-NPPA-D1S975 (cYS1173)-D1S968 (cYS1121)-D1S1062 (cYS73)-D1S1092 (cYS1148)-D1S1130 (cYS1232)/D1S1028 (cYS1363)-D1S967 (cYS1120)-PAX7-D1S1111 (cYS1180)-D1 S1073 (cYS191)-D1S1037 (cYS1406)-D1S1112 (CYS1181)-D1S1040 (cYS144)-D1S112-cen. The present FISH physical mapping within 1p36 will provide an useful information for hunting tumor suppressor genes suspected to locate on this region. To investigate whether there is a common genetic abnormality in various cases harbouring 1p36 chromosome translocation, we first assessed the breakpoints within 1p36 at three of in vitro established
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cell lines and two of in vivo cell lines maintained in SCID mouse. BALL-1, a B-cell line derived from an acute lymphoblastic leukemia patient, possesses an derivative chromosome of der(1)(?:1:?)(?::1p36-1q12::?). FISH analysis have revealed that the short arm of the derivative chromosome 1 is constructed by insertion of unknown fragment at the region of 1p36.21-36.22. The possible break points were mapped between markers D1S96 and D1S989 at the telomeric side and between D1S975 and D1S968 at the centromeric side. Further, cosmid markers D1S989 and D1S1131 were translocated to the other marker chromosome but NPPA and D1S975 were deleted from the derivative one. This suggests that complicated chromosome rearrangements have occurred in this cell line. All of the five cell lines surveyed have showed different breakpoints at cytogenetic level. However the breakpoint of HMS, an in vivo cell line maintained in SCID mouse, was mapped between markers D1S96 and D1S989 corresponding to the telomeric break end which is detected in BALL-1. Molecular screening of the gene locate on the breakpoint is now on going. Less
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