| Project/Area Number |
10670195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TANGE Tsuyoshi University of Tokyo, Faculty of Medicin, Associate Professor, 医学部附属病院, 助教授 (10107667)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Megakaryocyte / Differentiation / Thrombopoietin / C-MPl / gold-colloid / Eectron microscope / c-MPL / 免疫電顕 / C-mpl / c-mpl / C-Mpl |
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| Research Abstract |
In our laboratory, immuno-electron microscopyc was applied to demonstrate the location of c-MPL (thrombopoietin receptor) in human megakaryocytes using anti-human c-MPL mouse monoclonal antibody in 1998.
For the first two years, fundamental technique of post-embedding gold colloid method 〓sing immuno-electron microscopy was much learned, while in vitro culture of megakaryocytes isolated from bone marrow was continued as already reported to obtain the cell sample for c-MPL study.
In conclusion, we failed in demonstrating the location of c-MPL by electro-microscopy, while some ultrastructure of the megakaryocytes were proved to be essential for cell differentiation leading to the production of proplatelets or platelets.
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