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Altcration of Protease expression phenotype of mast cells by changing the microenvironment

Research Project

Project/Area Number 10670203
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionOsaka University

Principal Investigator

JIPPO Tomoko  Osaka University Medical School, Assistant Professor, 医学系研究科, 助手 (70252658)

Co-Investigator(Kenkyū-buntansha) KITAMURA Yukihiko  Osaka University Medical School, Professor, 医学系研究科, 教授 (70028520)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsmast cell / gene malker / protease / gene expression / cell differentiation / in situ hybridization / cell proliferation / mast cell-deficient mouse / 遺伝子表現 / in situ ハイブノタイゼーション / マウスの系統差 / マスト細胞欠損私物
Research Abstract

Mast cell proteases play an important role in the immediate hypersensitivity reaction. We studied the factors that could influence the expression of mouse mast cell protease (MMCP). By in situ hybridization, we showed that the MMCP-2 mRNA was expressed by mast cells located in the mucosa of the stomach. In contrast, mast cells located in the muscularis propria did not express the MMCP-2 mRNA. Next, we investigated changes in protease expression phenotype of mast cells after transplantation of bone marrow-derived cultured mast cells (BMCMCs) or peritoneal mast cells (PMCs) of WBB6FィイD21ィエD2-+/+ mice into tissues of mast cell-deficient WBB6FィイD21ィエD2-W/WィイD1VィエD1 mice. When 10ィイD15ィエD1 cells were injected, BMCMCs can adapt to new environments, whereas PMCs did not adapt. In contrast, when 20 PMCs were injected, they proliferated and adapted to the environment. Furthermore, we showed that the transcription factor MITF was involved in the transactivation of the MMCP-4, MMCP-5 and MMCP-6 genes. Since mast cell proteases are a mediator in the numerous allergic diseases, this study may provide us with a useful therapeutic strategy for allergic diseases.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Young-Mi dee et al.: "Alteration of protease expression phenotype of mouse peritoneal mast cells by changing the microenvironment as demonstrated by in situ hybridization histochemistry"American journal of Pathology. 153・3. 931-936 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A Ito et al.: "Systematic method to obtain novel genes that are regulated mi transcription factor : impaired expression of qranzyms B and thyptophan hydroxylase in mi/mi cultured must cells"Blood. 91・9. 3210-3211 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Dae-ki Kim et al.: "Impaired expression of integrin α-4 sabunit in cultured mast cells derived from mutant mice of mi/mi genetype"Blood. 92・6. 1973-1980 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K Tsujno et al.: "Pelayed onset of hemolytic anemia in CBA-pk-1soc/pk-1 sec mice with appoint mutation of the gene encoding red blood cell type pyrurate kinase"Blood. 91・6. 2169-2174 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A Ito et al.: "Inhibitory effect of the transcription factor encoded by the mi mutant allele in cultured must cells of mice"Blood. 93・4. 1189-1196 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T Jippo et al.: "Deficient transcription of mouse must cell protease 4 gene in mutant mice of mi/mi genotype"Blood. 93・6. 1942-1950 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y Kitamura et al.: "Signal transduction in mast cells and basophils"Razin/Rirera (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Lee YM, Jippo T, Kim DK, Katsu Y, Tsujino K, Morii E, Kim HM, Adachi S, Nawa Y, Kitamura Y: "Alteration of protease expression phenotype of mouse peritoneal mast cells by changing the microenvironment as demonstrated by in situ hybridization histochemistry."Am J Pathol. 153. 931-936 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ito A, Morii E, Maeyama K, Jippo T, Kim DK, Lee YM, Ogihara H, Hashimoto K, Kitamura Y, and Nojima H: "Systematic method to obtain novel genes that are regulated by mi transcription factor : impaired expression of granzyme B and tryptophan hydroxylase in mi/mi cultured mast cells."Blood. 91. 3210-3221 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsujino K, Kanno H, Hashimoto K, Fujii H, Jippo T, Morii E, Lee YM, AsaiH, Miwa S, and Kitamura Y: "Delayed onset of hemolytic anemia in CBA-Pk-lィイD1slcィエD1/Pk-lィイD1slcィエD1 mice with a point mutation of the gene encoding red blood cell type pyruvate kinase."Blood. 91. 2169-2174 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kim HM, Shin HY, Lee EN, Lee JE, An NH, Lee YM, Kim DK, Jippo T, and Kitamura Y: "Inhibition of immediate type allergic reactions by the aqueous extract of kum-hwnag-san."Int J Immunopharmacol. 20. 285-294 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kim DK, Morii E, Ogihara H, Hashimoto K, Oritani K, Lee YM, Jippo T, Adachi S, Kanakura Y, and Kitamura Y: "Impaired expression of integrin alpha-4 subunit in cultured mast cells derived from mutant mice of mi/mi genotype."Blood. 92. 1973-1980 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ito A, Morri E, Kim DK, Kataoka TR, Jippo T, Maeyama K, Nojima H, Kitamura Y: "Inhibitory effect-of the transcription factor encoded by the mi mutant allele in cultured mast cells of mice."Blood. 93. 1189-1196 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Jippo T, Lee YM, Katsu Y, Tsujino K, Morii E, Kim DK, Kim HM, Kitamura Y: "Deficient transcription of mouse mast cell protease 4 gene in mutant mice of mi/mi genotype."Blood. 93. 1942-1950 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kim DK, Morii E, Ogihara H, Lee YM, Jippo T, Adachi S, Kim HM, Kitamura Y: "Different effect of various mutant MITF encoded by mi, MiィイD2orィエD2 or MiィイD2whィエD2 allele on phenotype of murine mast cells."Blood. 93. 4179-4186 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kim HM, Kim YW, Lyu YS, Won JH, Kim DK, Lee YM, Morii E, Jippo T, Kitamura Y, An NH: "Inhibitory effect of mast cell-mediated immediate-type allergic reactions by Cichorium intybus."Pharmacol. Res. 40. 61-65 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Young-Mi dee et al.: "Alteration of protease expression phenotype of mouse peritonoal mast cells by changing the microenvironment as demonstrated by in situ hybridization histochemistry"American Journal of Pathology. 153・3. 931-936 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] A Ito et al.: "Systematic method to Obtain novel genes that are regulated mi transcription factor: impaired expression of granzyme β and tryptophan hydroxylase in mi/mi cultured mast cells"Blood. 91・9. 3210-3221 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Dae-Ki Kim et al.: "Impaired expression of integrin α-4 subunit in cultured mast cells derived from mutaut mice of mi/mi genotype"Blood. 92・6. 1973-1980 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Tsujno et al.: "Delayed onset of hemolytic anemia in CBA-pk-1^<slc>/pk-1^<slc> mice with a point mutation of the gene encoding red blood cell type pyruvate kinase"Blood. 91・6. 2169-2174 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] A.Ito et al.: "Inhibitory effect of the transcription factor encoded by the mi mutant allele in cultured mast cells of mice"Blood. 93・4. 1189-1196 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Jippo et al.: "Deficient transcription of mouse mast cell protease 4 gene in mutant mice of mi/mi genotype"Blood. 93・6. 1942-1950 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Kitamura et al.: "Signal transduction in mast cells and basophils"Razin/Rivera (in press.).

    • Related Report
      1999 Annual Research Report
  • [Publications] Young-Mi Lee et al: "alteration of protease expression phenotype of mouse porituneal mast cells by changing the microenrironment as demonstrated by in situ hypridization histochemislly" American Jounal of Pathology. 153・3. 931-936 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A Ito et al: "Systemic method it obtain novel genes that are regulated by an transcription factor:Impaired expression of granpyma B and tryptophin hydroxylase in mi/mi cultured mast cells" Blood. 91・9. 3210-3221 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Pacti Kim et al: "Impaired expression of Integrin α-4 subunit in cultured mast cells derived from mutant mice of mi/mi genotype" Blood. 92・6. 1973-1980 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Tsujino et al: "Delayed onset of tiomolytic ancmia in CBA-pk-l^<SM>/pk-l^<SM>mico with a point mutation at the gene encoding red Blood cell type pyruvoto riness" Blood. 91・6. 2167-2174 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A Ito et al: "Inhibitiry effeet of the transcription factor encoded by the mi mutant allelc in cultuicd mast cells of miss" Blood. in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] T Jippo.et al: "Deficient transcription of mouse most cell protrase 4 gene in mutant of mi/mi genotype" Blood. in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Kitamura et al: "signal transdvation in mast cells and basophils" Razin/Rirera in press,

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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