| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
The T-cell receptor (TCR)/CD3 complex on the surface or T-lymphcytes is required for recognition of the antigens presented by macrophages. Usually, such antigens are presented on Major Histocompatibility Complex (MHC) molecules. In this recognition system, extracellular domains of both TCR α and β chains are reported to be essential for antigen recognition.
On the other hand, it has been reported that the antigenicities of HTLV-I and -II producers, which are initially T-cells, change during cultivation, for example loosing some T-cell antigens or obtaining HLA-DR and/or CD25 antigens, as also reported in cases of high grade T-cell malignancy.
In the present study, immunoelectron microscopic observation was performed to clarify TCR/CD3 complex expression on the surface of human T-lymphotropic virus (HTLV) type I- and type II-producing cells. Staining with WT-31 (TCR α/β) was found to be positive only in PM, while staining for αF1 (TCR α) and for βF1 (TCR β) was positive only in NM and rER
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. CD3, on the other hand, reacts with both NM/rER and PM.
Using these antibodies, normal fresh, and fresh and short-term cultured HTLV-I carrier lymphocytes were clearly shown to possess TCR/CD3 complexes, lacking TCR α/β complex in particular, suggesting that their recognition of antigens is defcctive.
The ultrastructure of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 (HHV-8) has not yet been fully elucidated, although some findings have been reported using primary effusion lymphoma (PEL) cell lines, KS-1, harboring no Epstein-Barr virus (EBV) coinfection. In the present study, detailed fine structural examination of KSHV/HHV-8 was performed after stimulation of the PEL-derived cell line KS-1 with 12-O-tetradecanoy1-phorbol-13-acetate (TPA) in vitro. While nuclear virus particles associated with no extracellular mature particles. KS-1 cells stimulated with TPA produced many extracellular mature particles as well as intranuclear particles, in addition to interesting tubulo-reticular structures and aggregated tubular structures in vesicles. The induced intranuclear particles were empty, doughnut shaped, and dense cored, with outer, and inner diameters of 100-110nm and 60-70nm, respectively. Dense-cored extracellular mature particles were 150-160nm in diameter, and some contained doughnut-shaped cores, together with a few megaloviruses, 260nm in outer diameter. These findings indicate that KS-1 cells treated with TPA can produce extracellular mature particles as well as intranuclear particles, which were proven to be KSHV/HHV-8. Less
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