| Project/Area Number |
10670222
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
ABE Kenji Nat. Inst. Inf. Dis., Pathology, Senior Researcher, 感染病理部, 主任研究官 (60130415)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Hepatitis G / Hepatitis G virus / HGV genotype / HGV type 4 / Myanmar / Vietnam / Molecular epidemiology / phylogenetic analysis / G型肝炎ウイルス(HGV)遺伝子 |
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| Research Abstract |
We recently identified a novel genotype of hepatitis G virus (HGV) in Southeast Asian countries. To characterize the viral genome in more detail, we cloned the full-length nucleotide sequence of the two different HGV isolates belonging to the novel genotype. These isolates were recovered from serum of Myanmarese (designated HGV-MY14) and Vietnamese (designated HGV-VT48) subjects, respectively. Both HGV isolates were composed of 9228 nucleotides (nt) and had a single open reading frame spanning 8529 nt and encoding 2843 amino acid (aa) residues. This isolate differed from previously reported HGV/GBV-C isolates consisting of type 1 through 3, by 13-15% of the nucleotide sequence and 4-6% of the amino acid sequence. Both isolates lacked a clearly identifiable core gene that had only 16aa residues. Based on phylogenetic analysis of the full-genome sequence, HGV-MY14 and VT48 isolates can be classified into the novel genotype as well as the result in the 5'-UTR sequence, and named this genotype, type 4.
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