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A novel neutrophil chemotactic cytokine : identification and functional characterization in malaria

Research Project

Project/Area Number 10670229
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionThe University of Tokushima

Principal Investigator

OHASHI Makoto  Tokushima University, Faculty of Integrated Arts and Sciences, Professor, 総合科学部, 教授 (40128369)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsNeutrophil / Cytokine / Malaria / Chemotoctic factor / Interferon / 活性酸素 / ペプチド / 感染防御 / Plasmodium
Research Abstract

Blood cell lysate from Plasmodium yoelii infected mice (7 days post-infection) showed an intense neutrophil chemotactic activity in dose-dependent fashion. Time course study revealed that the neutrophil chemotactic activity of the red cell lysate increased prior to the increase the number of infected erythrocytes. Sequence analysis of NH_2-terminal amino acid of the purified NCF from red blood cell lysate showed that the NCF is a kind of interferon-induced protein (IP18). Mapping study indicates that the epitope for neutrophil chemotactic activity exists on the central part of the molecule. Using a polyclonal antibody to IP18, time course study was performed for detecting the change of the amount of IP18 on red blood cells during the P.yoelii infection. Although slight amount of IP18 was detectable on RBC from normal mouse, the amount of IP18 was remarkably increased and peaked at day 6 after P.yoelii infection. The amount of IP18 and its mRNA was also increased in splenocytes. When IP18 was added in the culture system of P.falciparum in the presence of various concentrations of neutrophils in vitro, the parasite growth was inhibited in dose dependent fashion of neutrophils. When ubiquitin was added instead of IP18, no inhibition of the parasite growth was observed so far as examined. These results indicate that IP18 likely acts as an important molecule for the host defense system against malaria infection.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Owhashi M,Harada M,Sogai S,Ohmae H,Ishii A: "The role of saliva of Anopheles stephensi in inflammatory response : Identification of a high molecular weight neutrophil Chemotactic factor"Parasitology Research. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Owhashi M,Arita H,Hayai N: "Identification of a novel eosinophil chemotactic lytokine (ECF-L) as a chitinase family protein"Journal of Biological Chemistry. 275. 1279-1286 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Owhashi M,Arita H,Niwa A: "Production of eosinophil chemotactic factor by CD8^<-1> Tcells in Toxocara camis-infected mice"Parasitology Research. 84. 136-138 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Owhashi M., Harada M., Suguri S., Ohmae H., and Ishii A.: "The role of Saliva of Anopheles stephensi in inflammatory response : Identification of a high molecular weight neutrophil chemotactic factor."Parasitology Research. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Owhashi M., Arita H.and Hayai N.: "Identification of a novel eosinophil chemotactic cytokine (ECF-L) as a chitinase family protein."Journal of Biological Chemistry. 275. 1279-1286 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Owhashi M., Arita H., and Niwa A.: "Production of eosinophil chemotactic factor by CD8^+ T cells in Toxocara canis-infected mice."Parasitology Research. 84. 136-138 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Owhashi M,Harada M,Suguri S,Ohmae H,Ishii A: "The role of saliva of Anopheles stephensi in inflammatory response : Identification of a high molecular weight neutrophil chemotactic factor"Parasitology Research. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Owhashi M,Arita H,Hayai N: "Identification of a novel eosinophil chemotactic cytokine (ECF-L) as a chitinase family protein"Journal of Biological Chemistry. 275. 1279-1286 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ouhashi M,Arita H.Haya:N: "Identification of a Novel chemotactic cytokine(ECF-L) as a chitimase Family protein"J.Biol.Chem. 275. 1279-1286 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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