Genetic and biochemical analysis of insecticide-insensitivity of acetylcholinesterase in vector mosquitoes

• Project/Area Number: 10670246
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 寄生虫学(含医用動物学)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TOMITA Takashi National Institute of Infectious Diseases, Department of Medical Entomology, Chief, 昆虫医科学部, 室長 (20180169)
• Co-Investigator(Kenkyū-buntansha): 河野 義明 国立感染症研究所, 昆虫医科学部, 室長 (10225386)
• Project Period (FY): 1998 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: acetylcholinesterase / Culex tritaeniorhynchus / insecticide resistance / organophosphate / linkage analysis / cDNA / protein polymorphism / 有機リン剤

Research Abstract

Protein polymorphism attributable to organophosphorus(OP) insecticide-insensitivity of acetylcholinesterase(AChE) was investigated with mosquitoes of three insecticide-suceptible strains and the OP-resistant strain, Toyama, and mosquitoes newly collected from the natural population in Yokohama of Culex tritaeniorhyncus (C.t.). All the 20 field mosquitoes tested contained OP-insensitive AChE as well as Toyama strain. No protein sequence polymorphism was detected in the deduced matured peptide of AChE in all the tested mosquitoes.
A comparative linkage map for the mosquito, Culex tritaeniorhynchus, was constructed based on restriction fragment length polymorphism (RFLP) markers using cDNA clones from Aedes aegypti and AChE cDNA from C.t. OP resistance is controlled by a major gene (AChE^R) on chromosome 2, while the AChE structural gene (Ace) is located on chromosome 1. No evidence for a second Ace gene was observed, suggesting that the AChE^R locus represents a modifier of insecticide-sensitivity of AChE.

Research Products

• [Journal Article] Comparative linkage map developement and identification of an autosomal locus for insensitive acetylcholinesterase-mediated insecticide resistance in Culex tritaeniorhynchus (2001)
  • Author(s): Mori A, Tomita T, Hido Q, Kono Y, Severson DW
  • Journal Title: Insect Molecular Biology 10(2)(in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparative linkage map development and identification of an autosomal locus for insensitive acetylcholinesterase-mediated insecticide resistance in Culex tritaeniorhynchus. (2001)
  • Author(s): Mori A, Tomita T, Hidoh O, Kono Y, Severson DW
  • Journal Title: Insect Molecular Biology 10(2)(in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mori A,Tomita T, Hidoh O,Kono Y,Severson DW: "Comparative linkage map development and identification of an autosomal locus for insensitive acetyl cholinesterase-mediated insecticide resistance in culex tritaeniorhyncus."Insect Molecular Biology. 10(2)(in press). (2001)