| Project/Area Number |
10670258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
TOMOCHIKA Ken-ichi Faculty of Pharmaceutical Sciences, Okayama University, Associate professor, 薬学部, 助教授 (00093691)
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| Co-Investigator(Kenkyū-buntansha) |
SHINODA Sumio Faculty of Pharmaceutical Sciences, Okayama University, Associate professor, 薬学部, 教授 (50029782)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | metalloprotease / hemorrhagic actions / Vibrio vulnificus / type IV collagen / dermis / dermatosis / 基底膜 / 皮膚病変 / 血管内皮細胞 |
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| Research Abstract |
Vibrio vulnificus is an opportunistic pathogen causing wound infections and septicemia, characterized by hemorrhagic and edematous damage to the skin. This human pathogen secretes a metalloprotease (V. vulnificus protease {VVP}) as an important virulence determinant. When several bacterial metalloproteases including VVP were injected intradermally into dorsal skin, VVP showed the greatest hemorrhagic activity. The level of the in vivo proteolytic activity of the bacterial metalloprotease was significantly correlated with that of the in vitro hemorrhagic activity for the reconstituted basement membrane gel. Of two major basement membrane components (laminin and type IV collagen), only type IV collagen was easily digested by VVP. Histopathological observations of skin injected with VVP indicated the severe damages in deep region of dermis and subcutaneous tissue. In these portion, hemorrhage and swelling of lipocyte (fat cell or adipose cell) were observed near the blood vessels in the dermis and subcutaneous tissues. The capillary vessels in hemorrhagic region were destroyed or congested with blood. Histopathological observations of the hemorrhagic actions induced by VVP indicated the another possibilities of the degradation of capillary vessels by the necrosis of endothelial cells.
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