| Project/Area Number |
10670262
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Institute of Tropical Medicine, Nagasaki University |
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Principal Investigator |
ICHINOSE Yoshio Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Assistant professor, 熱帯医学研究所, 講師 (70176296)
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| Co-Investigator(Kenkyū-buntansha) |
AKAIKE Takaaki Department of Microbiology, School of Medecine, Kumamoto University, Associate professor, 医学部, 助教授 (20231798)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Vibrio cholerae non-01 / metalloprotease / invasive mechanism / Vibrio cholerae / invasive factor |
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| Research Abstract |
There are many reports on the cases of Vibrio cholerae infection which may extend to extra-intestinal organs. It can result in sepsis and multiple organ failure especially in immunocompromised hosts. It is then considered that a metalloprotease produced by Vibrio cholerae may be involved in its invasive mechanisms of vibrios into tissues or vessels. Because it is a permeability factor to activate kinin cascade and an activating factor of a matrix metalloprotease produced by leukocytes migrating in the inflammatory lesion. We were then trying to develop an animal model of sepsis due to Vibrio cholerae 019 to elucidate the invasive mechanisms of Vibrio cholerae. The results obtained through the study on the role of metalloprotease in pathogenic mechanisms of Vibrio cholerae and histopathological findings in sepsis model are as follows ;
(1)Improvement of purification method of choleraprotease.
(2)Establishment of assay system of choleraprotease using immunoblotting with ECL kit.
(3)Analysis of nicking site of cholera toxin by choleraprotease.
(4)Development of mouse sepsis model using Vibrio cholerae 019.
(5)Involvement of choleraprotease in the pathogenic mechanism of sepsis.
(6)Involvement of iNOS in the pathogenic mechanism of sepsis.
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