| Project/Area Number |
10670265
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | AKITA PREFRCTYRAL UNIVERSITY (1999)
Yokohama City University (1998) |
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Principal Investigator |
FUKUSHIMA Jun Akita Pref. Univ., Dept. of Biotechnology, Associate Prof., 生物資源科学部, 助教授 (00181256)
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| Co-Investigator(Kenkyū-buntansha) |
KUDOH Ichidai Yokohama City University, Sch. of Medicine, Associate Professor, 医学部, 助教授 (30145700)
浜島 健治 横浜市立大学, 医学部, 助手 (00114611)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Pseudomonas aeruginosa / DNA immunization method / flagellar antigen / cellular immunity / delayed type hypersensitivity / crytotoxic T lymphocyte / pilli antigen / protective immunity / 緑膿菌 / IL-2発現プラスミド / 感染防御 / Pseudomonas aeruginosa / DNAワクチン / サイトカイン / アジュバント / CpGモチーフ |
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| Research Abstract |
It will become more important to protect the Pseudomonas aeruginosa infection in the future. There is no effective vaccine for P. aeruginosa lung infection. We intend to develop the vaccine against infection by the novel method using eukaryotic expression plamid containing the gene of P. aeruginosa antigens. Firstly, I developed the flagellin gene vaccine which containing cytomegarovirus promoter. We could get the systemic responses in the humoral and cellular immunity by the injection of this plasmid solution into the mouse nasal cavity.
In the experiment of mouse infection model, we observed certain level of protection of lung infection. Next, to get more effective protection, the pilin expression plasmid was constructed. IL-2 expression plasmid was co-injected with antigen plasmid to get better cellular and protective immunity. The good immune responses were obtained by the pilin plasmid infection and cellular immunity was enhanced by the co-injection of IL-2 plasmid nevertheless humoral immunity was not altered. In the future research of DNA immunization, effective vaccine against P. aeruginosa lung infection might be developed.
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