| Project/Area Number |
10670273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Nippon Medical School |
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Principal Investigator |
OHKUNI Hisashi Institute of Gerontology, Nippon Medical School, Professor, 老人病研究所, 教授 (60060365)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANAMI Ohichi Institute of Gerontology, Nippon Medical School, Professor, 老人病研究所, 教授 (70096973)
SAKURADA Shinsaku Institute of Gerontology, Nippon Medical School, Assistant, 老人病研究所, 助手 (50178620)
TODOME Yuko Institute of Gerontology, Nippon Medical School, Assistant Professor, 老人病研究所, 講師 (20089626)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | viridans group streptococci / S. mitis / extracellular products / acute inflammation / vasculitis / cytokines / NF-x B / NF-κB |
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| Research Abstract |
We attempted to demonstrate the nuclear translocation of transcription factor, NF-x B in cultured human umblical vein endotherial cells (HUVEC) and in human monocytes reacted with the extracellular products from Streptococcus mitis and the heat-killed whole cells. Cytokines released from the ECP-or the whole cells-stimulated HUVEC and monocytes were also assessed.
The results obtained were as follows.
1) The ECP exhibited strong cytotoxicactivity to HUVEC, and did not induce the nuclear translocation of NF-x B in HUVEC. The ECP could not also induce the release of TNF-α, IL-1β and IL-6 from HUVEC.
2) The ECP did not activate NF-x B in monocytes/macrophages, but induced the release of TNF-α, IL-1β and IL-6 from monocytes/macrophages.
3) The heat-killed whole cells induced the nuclear translocation of NF-x B, the expression of mRNA of cytokines as TNF-α, IL-1β and IL-6 in monocytes/macrophages, and the release of these cytokines.
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