| Project/Area Number |
10670284
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
TSUTSUI Yoshihiro Hamamatsu University School of Medicine, 2nd Department of Pathology, Professor, 医学部, 教授 (50073135)
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| Co-Investigator(Kenkyū-buntansha) |
KOSUGI Isao Hamamatsu University School of Medicine, 2nd Department of Pathology, Reseach Associate, 医学部, 助手 (10252173)
BABA Satoshi Hamamatsu University School of Medicine, 2nd Department of Pathology, Assosiate Professor, 医学部, 助教授 (10242760)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Cytomegalovirus / Apoptosis / neuronal cell death / Intrauterine infection |
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| Research Abstract |
Cytomegalovirus (CMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, we found that apoptosis is induced in the developing mouse brain infected with murine cytomegalovirus (MCMV) in an association with neuronal cell loss. With the combination of the terminaldeoxynucleotidyl transferase-mediated dUTP nick edn labeling (TUNEL) technique and immunohistochemical staining. A few percent TUNEL-positive cells were double-labeled with neuronal glial markers, while more than 30% of microglial cells were turned into apoptosis, presumably the consequence of engulfment of the neuronal apoptotic cells. In the primary neuronal cultures, MCMV infection inhibited the induction of apoptosis either by serum deprivation or glutamate treatment. The induction and blocking of neuronal apoptosis by virus infection may be important for morphological and functional disorders in the congenital CMV infection.
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