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Analysis of antigenic properties of human herpesvirus 7 and the host immune responses

Research Project

Project/Area Number 10670285
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionOkayama University

Principal Investigator

YAMADA Masao  Medical School, Okayama University, Professor, 医学部, 教授 (40166731)

Co-Investigator(Kenkyū-buntansha) ISOMURA Hiroki  Medical School, Okayama University, Assistant, 医学部, 助手 (20294415)
NAMBA Hikaru  Medical School, Okayama University, Assistant, 医学部, 助手 (20273972)
YOSHIDA Mariko  Medical School, Okayama University, Lecturer, 医学部, 講師 (20144743)
OHUCHI Reiko  Medical School, Okayama University, Assistant, 医学部, 助手 (00304311)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordshuman herpesvirus / dot-blot-neutralization assay / seroepidemiology / transferred antibody / seroconversion / monoclonal antibody / phosphoprotein / リン酸化蛋白 / ドットブロット中和抗体測定法 / HHV-6,-7の血清疫学
Research Abstract

To elucidate antigenic properties of human herpesviruses 6 and 7 (HHV-6 and -7), three monoclonal antibodies (Mabs) against HHV-6 and 13Mabs against HHV-7 were established and characterized by radio-immunoprecipitation. The 40-kDa phosphoprotein by recognized by a Mab (TK17) was the product of HHV-7 U27 gene. Among these Mabs, a Mab(IK3) which recognizes the 135-kDa late polypeptide of HHV-6 and several Mabs (TK3 and others) which recognize the 125-kDa late polypeptide of HHV-7 were selected to monitor virus growth by a dot blot antigen-detection method. Using the dot blot method, we established a neutralizing antibody assay for these viruses. The dot-blot neutralization assay is easy to perform, is highly reproducible and objective when compared with the conventional methods based on cytopathology or immunofluorescence for determining neutralization endpoints. Using this method, 55 sera from healthy adults were examined. In most individuals, neutralizing antibody titers against HHV-7 were much higher than those against HHV-6. Elevated neutralizing titers against HHV-7 might be attributed to continuous booster effects by persistent HHV-7 production in saliva. Furthermore, we monitored neutralizing antibody titers against these viruses in 15 children with documented history of exanthem subitum. Transferred antibody titers against these viruses from mothers were readily demonstrated by the neutralization test. Transferred antibody titers against HHV-7 were higher and remained longer after birth than those of HHV-6, and these findings were in accord with clinical observation that HHV-6 infection usually occurs earlier than HHV-7 infection. It is notable that no cross-reactive elevation of heterologous neutralizing antibody titer was observed.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Tsukazaki, T.他5名: "Development of a dot-blot neutralizing assay for HHV-6 and -7 using specific monoclonal antibodies"J. Virol. Methods. 73. 141-149 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshida, M.他6名: "Comparison of antiviral compounds against human herpesvirus-6 and -7"Antiviral Res.. 40. 73-84 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kuzuya, M.他12名: "Survey of human group C rotaviruses in Japan during the winter of 1992 to 1993"J. Clin. Microbiol.. 36. 6-10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山田雅夫: "ヒトヘルペスウイルス6と7の比較生物学"ウイルス. 48. 39-44 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山田雅夫: "ウイルスの潜伏感染と活性化 1.単純ヘルペスウイルスとその患者"治療学. 32. 597-601 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山田雅夫: "ヘルペスウイルス6・7感染のトロピスム"潜伏・持続感染 微生物と宿主の反応 第3巻「HHV-6、7の潜伏感染」(倉田、天野編)菜根出版,東京,. 107-116 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maeda, Y.他8名: "Monitoring of human herpesvirus after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation"Brit. J. Haematol.. 105. 295-300 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhu, H.-Y.他4名: "Intracellular localization of the UL31 protein of herpes simplex virus type 2"Arch. Virol.. 144. 1923-1935 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii, R. 他5名: "Neutralization assay for human group C rotavirus using a reverse passive hemagglutination test for endpoint determination"J. Clin. Microbiol.. 38. 50-54 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Isomura, H.他8名: "Suppressive effects of human herpesvirus 6 on thrombopoietin-inducible megakaryocytic colony formation in vitro"J. Gen. Virol.. 81. 663-673 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Goshima, F. 他6名: "Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells"Arch. Virol.. 145. 417-426 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujiwara, N.他6名: "Monitoring of human herpesvirus-6 and -7 genomes in saliva samples of healthy adults by competitive quantitative PCR"J. Med. Virol.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山田雅夫: "ヘルペスウイルス感染-初感染、潜伏感染、回帰発症-"臨床と研究. 76. 6-10 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 山田雅夫: "第8章 臓器感染症:皮膚粘膜系,泌尿生殖系,神経系 コンパクト微生物学,小熊惠二,東匡伸編"南江堂、東京(分担部分:PP148-152,172-177,178-183). 209 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsukazaki T. and 5 authors: "Development of a dot-blot neutralizing assay for HHV-6 and -7 using specific monoclonal antibodies"J. Virol. Methods.. 73. 141-149 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshida M. and 6 authors: "Comparison of antiviral compounds against human herpesvirus -6 and -7"Antiviral Res.. 40. 73-84 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kuzuya, M. and 12 authors: "Survey of human group C rotaviruses in Japan during the winter of 1992 to 1993"J. Clin. Micrbiol.. 36. 6-10 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada, M: "Comparative biology of human herpesviruses 6 and 7. (in Japanese)"Uirusu. 48. 39-44 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada, M: "Viral latency and reactivation. 1. Herpes simplex viruses and diseases caused by them. (in Japanese)"Chiryo-gaku. 32. 597-601 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada, M: "Tropism of human herpesviruses -6 and -7 infection. (in Japanese)"Latent and persistent pathogens, vol.3 Latent infection with HHV-6 and -7 (eds. Kurata, Amano) Saikon Press Tokyo. 107-116 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maeda, Y. and 18 authors: "Monitoring of human herpesviruses after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation"Brit. J. Haematol.. 105. 295-302 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhu, H. -Y. and 4 authors: "Intracellular localization of the UL31 protein of herpes simplex virus type 2"Arch. Virol.. 144. 1923-1935 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii, R. and 5 authors: "Neutralization assay for human group C rotaviruses using a reverse passive hemagglutination test for endpoint determination"J. Clin. Microbiol.. 38. 50-54 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Isomura, H. and 8 authors: "Suppressive effects of human herpesvirus 6 on thrombopoietin-inducible megakaryocytic colony formation in vitro"J. Gen. Virol.. 81. 663-673 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Goshima, F. and 6 authors: "Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells"Arch. Virol.. 145. 417-426 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujiwara, N. and 6 authors: "Monitoring of human herpesvirs-6 and -7 genomes in saliva samples of healthy adults by competitive quantitative PCR"J. Med. Virol.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada, M.: "Herpesvirus infection --- primary infection, latent infection and recrudescence --- (in Japanese)"Rinshyo to Kenkyu. 76. 6-10 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada, M.: "Compact Microbiology. Chapter 8, Infectious diseases by organ system ; mucocutaneous, urogenital and nervous systems"Nankodo, Tokyo. 209,148-152,172-177,178-183 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maeda, Y. 他 18名: "Monitoring of human herpesviruses after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation"Brit. J. Haematol.. 105. 295-305 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zhu, H,-Y. 他 4名: "Intracellular localization of the UL31 protein of herpes simplex virus type 2"Arch. Virol.. 144. 1923-1935 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujii, R. 他 5名: "Neutralization assay for human group C rotaviruses using a reverse passive hemagglutination test for endpoint determination"J. Clin. Microbiol.. 38. 50-54 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Isomura, H. 他 8名: "Suppressive effects of human herpesvirus 6 on thrombopoietin-inducible megakaryocytic colony formation in vitro"J. Gen. Virol.. 81. 663-673 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Goshima, F. 他 6名: "Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells"Arch. Virol.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujiwara, N. 他 6名: "Monitoring of human herpesvirus-6 and -7 genomes in saliva samples of -healthy by competitive quantitative PCR"J. Med. Virol.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 山田雅夫: "ヘルペスウイルス感染 初感染、潜伏感染、回帰発症"臨床と研究. 76. 6-10 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 山田雅夫 (分担): "コンパクト微生物学,小熊恵二,東匡伸編 第8章 臓器感染症:皮膚粘膜系,泌尿生殖系,神経系"南江堂 (内 20ページ担当). 207 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsukazaki T. 他5名: "Deveropment of a dot-blot neutralizing assay for HHV-6 and -7 using specific monoclonal antibodies." J.Virol.Methods. 73. 141-149 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida M. 他6名: "Comparison of antiviral compounds against human herpesvirus-6 and-7." Antiviral Res.40. 73-84 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kuzuya, M. 他12名: "Survey of human group C rotaviruses in Japan during the winter of 1992 to 1993" J. Clin. Micrbiol.36. 6-8 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山田雅夫: "ヒトヘルペスウイルス6と7の比較生物学" ウイルス. 48. 39-44 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山田雅夫: "ウイルスの潜伏感染と活性化 1.単純ヘルペスウイルスとその疾患" 治療学. 32. 597-601 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山田雅夫: "ヘルペスウイルス6・7感染のトロピズム" 潜伏・持続感染 微生物と宿主の反応 第3巻「HHV-6、7の潜伏感染」(倉田、天野編)菜根出版,東京,. 107-116 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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