• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

STRUCTURE-FUNCTION RELATIONSHIP OF VIRAL RNA DEPENDENT RNA POLYMERASE.

Research Project

Project/Area Number 10670291
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionKurume University

Principal Investigator

TOYODA Tetsuya  KURUME UNIVERSITY, SCHOOL OF MEDICINE, VIROLOGY, PROFESSOR, 医学部, 教授 (00197972)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsINFLUENZA VIRUS / HEPATITIS C VIRUS / RNA POLYMERASE / SUBUNIT / PROTEASE / RNase / キャップ
Research Abstract

The RNA-dependent NA polymerase of influenza A virus consists of three subunits, pB1, PB2 and PA. PB1 is a catalytic subunit of NA synthesis. PB2 confers a transcriptase activity to PB1. PA provides replicase activity and performs a recently identified unique protease activity. In order to map its functional domains, we generated site-specific antibodies. RNA synthesis activity was mapped in amino acids 1-159 of PB2 and 501-617 of PA. Cap-I dependent RNase activity was mapped in amino acids 1-159 and 305-559 of PB2, 1-222 of PB1, and 601-716 of PA. Cap-binding site was mapped in amino acids 402-559 of PB2.
We previously determined that the C-terminal 158 amino acids of PB1 bound to the N-termina1 249 amino acid stretch of PB2, while the N-terminal 140 amino acids ofPB1 bound to the C-terminal two third region of PA. This time, we have narrowed the PB1-PB2 binding site in 28 residues of PB2 and 180 residues of PB1, and the PB1-PA binding site in 25 residues of PB1 and about 200 residues of PA.
In order to expand our knowledge into other viral RNA-dependent RNA polymerases, we established in vitro transcription system of hepatitis C RNA polymerase.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] N.Hamada: "Nucleotide sequence of the gene encoding the RNA polymorase and the 3' non-coding region of a bovine enterovirus Japanese isolate : Rapid synonymous substitutions between European and Japanese strains"Arch. Virol.. 143. 815-821 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shoji Kaminaka: "Studies of Bovine Enterovirus Structure by Ultraviolet Resonance Raman Spectroscopy"J. Virol. Methods. 77. 117-123 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kenji Masunaga: "Molecular mapping of influenza virus RNA polymerase by site-specific antibodies"Virology. 256. 130-141 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroyuki Masuda: "Subsitution of amino acid residue in influenza A virus hemagglutinin affects recognition of glycosphingolipid containing N-g1ycolylneuraminic acid"FEBS LETT. 464. 71-74 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toyoda T.: "Molecular dissection of influenza virus RNA polymerase"Rec. Res. Dev. Virol.. 1(印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katsuro Tsuji: "No association of Borna disease virus with psychiatric disorders among patients in Northern Kyushu, Japan"J. Med. Virol.. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toyoda T.: "Antibody-scanning and epitope-tagging methods ; Molecular mapping of proteins using antibodies"Curr. Prot. Pept. Sci.. 1(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 豊田哲也: "インフルエンザウイルスRNAポリメラーゼサブユニット(PBI、PB2、PA)の構造と機能相関"日本臨床. 55. 2593-2598 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 豊田哲也: "インフルエンザウイルスRNAポリメラーゼの分子解剖 II-構造と機能相関-"ウイルス. 47. 155-164 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 豊田哲也: "たかが風邪、されど風邪"日本医事新報ジュニア版. 379. 7-10 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 豊田哲也: "インフルエンザの構造と遺伝子"化学療法の領域. 15. 41-46 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 豊田哲也: "感染分子機構から見た治療薬開発の方向性"治療学. 34. 44-49 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 豊田哲也: "ウイルス学"朝倉書店. 646 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Hamada et al.,: "Nucleotide sequence of the gene encoding the RN'A polymerase and the 3, non-coding region of a bovine enterovirus Japanese isolate : Rapid synonymous substitutions between European and Japanese strains."Arch. Virol.. 143. 815-821 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shoji Kaminaka et al.,: "Studies of Bovine Enterovirus Structure by Ultraviolet Resonance Raman Spectroscopy."J. Virol. Methods. 77. 117-123 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kenji Masunaga et al.,: "Molecular mapping of influenza virus RNA polymerase by site-specific antibodies."Virology. 256(1). 130-141 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroyuki Masuda et al.,: "Substitution of amino acid residue in influenza A virus hemagglutinin affects recognition of glycosphingolipid containing N-glycolylneuraminic acid.,"FEBS LETT. 464. 71-74 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katsuro Tsuji et al.,: "No association of Borna disease virus with psychiatric disorders among patients in Northern Kyushu, Japan."J. Med. Virol.. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuya Toyoda et al.,: "Molecular dissection of influenza virus RNA polymerase."Rec. Res. Dev. Virol. 1 in press. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuya Toyoda: "Antibody-scanning and epitope-tagging methods ; Molecular mapping of proteins using antibodies."Curr, Prot. Pept. Sci. 1 in press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuya Toyoda: "Structure-function relationship of the influenza virus RNA polymerase subunits."Jpn. J. Clin. Med.. 55(10) in Japanese. 95-100 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuya Toyoda: "Molecular dissection of the influenza virus RNA polymerase. II. : Structure-function relationship"Uirusu. 47(2)in Japanese. 155-164 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuya Toyoda: "Flu and Cold"Jpn. Med. J. Jr.. 379 in Japanese. 7-10 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuya Toyoda: "Molecular structures of influenza virus."Antibiotics & Chemotherapy. 15(10) in Japanese. 41-46 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuya Toyoda and Koyu Hara: "A new strategy for development of anti-viral reagents"Biomedicine & Therapeutics. 34(1) in Japanese. 44-48 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuya Toyoda and Akira Ishihama: "Orthomyxoviridae In Virology, Hatanaka, M. Ed."Asakura Shoten (in Japanese). 230-243 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shoji Kaminaka: "Studies of Bovine Enterovirus Structure by Ultraviolet Resonance Raman Spectroscopy."J.Virol.Methods. 77. 117-123 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kenji Maseunaga: "Molecular mapping of influenza virus RNA polymerase by site-specific antibodies."Virology. 256. 130-141 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hiroyuki Masuda: "Subsitution of amino acid residue in influenza A virus hemagglutinin affects recognition of glycosphingolipid containing N-glycolylneuraminic anid."REBS LETT.. 464. 71-74 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuro Tsuji: "No association of Borna disease virus with psychiatric disorders among patients in Northern Kyushu,Japan."J.Med.Virol.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 豊田哲也: "たかが風邪、されど風邪"日本医事新報ジュニア版. 379. 7-10 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 豊田哲也: "インフルエンザの構造と遺伝子"化学療法の領域. 15. 41-46 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 豊田哲也: "感染分子機構から見た治療薬開発の方向性"治療学. 34. 44-49 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kenji Masunaga: "Molecular mapping of influenza virus RNA polymerase by site-specific antibodies." Virology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Shoji Kaminaka: "Studies of Bovine Enterovirus Structure by Ultraviolet Resonance Raman Spectroscopy." J.Virol.Methods. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Hamada: "Nucleotide sequence of the gene encoding the RNA polymerase and the 3′non-coding region of a bovine enterovirus Japanese isolate:Rapid synohymous substitutions between European and Japanese strains." Arch.Virol.143. 815-821 (1998)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi