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Genetic analysis of stress effects on cutaneous immune system

Research Project

Project/Area Number 10670377
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Public health/Health science
Research InstitutionOsaka Prefectural Institute of Public Health

Principal Investigator

NAKANO Yumiko  Osaka Prefectural Institute of Public Health, Occupational Health, Chief Research Worker, 労働衛生部, 主任研究員 (40159067)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsStress / Contact sensitivity / Langerhans cell / Keratinocyte / isolation / adhesion molecule / cytokine / neuropeptide / 慢性的精神的ストレス / 遺伝子発現解析 / RT-PCR法 / アレルギー性接触皮膚炎 / 単独隔離 / 接着分子 / サイトカイン / 神経ペプチド
Research Abstract

Although stress is thought to worsen inflammation diseases, the mechanism is not known. Therefore, long period of isolation was administered to BALB / c male mouse as chronic mental stress, and the influence on contact dermatitis (CS) and function of Langerhans cells (LC) and keratinocytes (KC) were analyzed. As a result, by chronic stress the CS responses and the antigen presenting ability of LC were markedly enhanced, while the antigen presenting ability, IL-1-α and TNF-α (inflammatory cytokine) production and proliferative activity of KC were drastically reduced. Then, RNA of KC from mice received isolation stress was extracted and mRNA expression for various genes was analyzed using RT-PCR method. As preliminary experiments mRNA expression by KC after stimulation with phenol, a primary irritant, and trinitrochlorobenzene (TNCB), a contact sensitizer, were analyzed. As a result, only adhesion molecule ICAM-1 mRNA expression was induced by the former, while marked expression of mRNA for ICAM-1, E-cadherin (adhesion molecule), transglutaminase (differentiation marker), c-fos and c-myc (immediate early genes), corticotrophin releasing hormone receptor and substance P receptor ( neurotransmitter related), and IL-1-α and TNF-α were observed by the latter. Chronic stress up-regulated these mRNA transiently. However, application of TNCB to chronically stressed mouse, only expression of mRNA for transglutaminase and substance P receptor by KC was increase, and mRNA expression for other genre was reduced compared to control. These results suggested that by chronic stress the function of LC is enhanced, while function and differentiation of KC are impaired and that substance P released from neurofibers is related to these modulations.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report

Research Products

(13 results)

All Other

All Publications (13 results)

  • [Publications] Nakano Y. et al: "Immune function and lifestyle of taxi drevers"Industrial Health. 36. 32-39 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakano Y.: "Antigen-presenting cell function of epidermal cells activated by hapten application"Brit J Dermatol. 38. 786-794 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 中野ユミ子: "ストレスは接触皮膚炎を増悪する"臨床免疫. 32. 365-371 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakano Y. and Nishimura H.: "Effect of isolation stress on cutaneous immune function"J Immunol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakano Y., Nakamura S., Hirata M., Harada K., Ando K., Tabuchi T., Matunaga I. and Oda H.: "Immune function and lifestyle of taxi drivers."Industrial Health. 36. 32-39 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakano Y.: "Antigen-presenting cell function of epidermal cells activated by hapten application."Brit J Dermatol. 38. 786-794 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yumiko Nakano: "Stress worsens contact sensitivity"Rinshyou Menneki. 32. 365-371 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakano Y. and Nishimura H.: "Effect of isolation stress on cutaneous immune function"J Immunol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Nakano: "Antigen-presenting cell function of epidermal cells activated by hapten application"Brit. J. Dermatol.. 138. 786-794 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中野ユミ子: "ストレスは接触皮膚炎を増悪する"臨床免疫. 32(3). 365-371 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Nakano: "Antigen-presenting cell function of epidermal cells activated by hapten application" Brit.J.Dermatol.138. 786-794 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Nakano et al: "Immune function and lifestyle of taxi drivers in Japan" Industrial Health. 36. 32-39 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Nakano et al: "Relationships between immune prameters and health status in middle-aged 1male Japanese workers" Industrial Health. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-03-31   Modified: 2016-04-21  

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