DNA typing without extracting DNA.

Research Project

Project/Area Number	10670381
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Legal medicine
Research Institution	Yamagata University
Principal Investigator	UMETSU Kazuo Yamagata University School of Medicine, Associate professor, 医学部, 助教授 (10091828)
Co-Investigator(Kenkyū-buntansha)	YUASA Isao Tottori University School of Medicine, Assistant professor, 医学部, 講師 (00093633) 鈴木庸夫山形大学, 医学部, 教授 (70004588)
Project Period (FY)	1998 – 2000
Project Status	Completed (Fiscal Year 2000)
Budget Amount *help	¥3,800,000 (Direct Cost: ¥3,800,000) Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000) Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000) Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywords	DNA typing / PCR / STR / DNA増幅 / PCR法 / DNA多型 / STR多型 / 直接検出法 / AmpliTaq Gold / 血液
Research Abstract	The extraction kit of DNA is marketed in great numbers, and the preparation of the sample becomes considerably easy. However, the PCR is the simplest, if it is completed by putting direct sample in generally using method. This time, the method that it did not extract DNA and does the direct PCR was examined. The blood right after blood collection was not amplified by the PCR.As this cause, DNA was fixed to cell, and it did not work as a template. However, it was possible to use as sufficiently sample, when it passes through the 2,3 day. STR typing was successfully applied to various samples such as serum, whole blood, saliva, nail, blood stain, urine and mustache without extracting DNA from them. The simple and fast methods for PCR as demonstrated herein will be useful in the field of forensic practice.