| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
A microsatellite D14S299 (wglc5) locus, in human has the variable repeats (GGAT or GGAA), and the nucleotide sequence polymorphisms in the Japanese population has been already reported. PCR amplification of the microsatellite at the D14S299 orthologue was performed with the same primers as used in human from 19 non-human primate species representing 3 species of ape, 8 of old World monkey, 6 of New World monkey and 2 of prosimian. PCR products were obtained from all species samples except prosimian. We determined nucleotide sequences of each allele in primates including 3 ape species (common chimpanzee, white-handed and agile gibbons) and 7 old World monkey species (baboon, Africa green monkey, patas monkey, stump-tailed macaque, crab eating macaque, rhesus macaque and Japanese macaque). The sequence data were compared with those of human. Three repetitive regions (GGAT or GGAA) in human were conserved among all alleles of catarrhine primates examined. The number of repetitive GGAT unit, which repeated only twice in human and common chimpanzee, increased as 3 to 11 in other catarrihine primates. Some species-or genus-specific nucleotide substitutions and/or insertions/deletions of tetranucleotide repeat unit were also observed. These results were summarized and published in DNA Polymorphism. in this year, the D14S299 orthologue was sequenced from three more ape species (pygmy chimpanzee, gorilla and orangutan). Form variable repeats as same as catarrhine primates were also observed in those species. A single nucleotide substitution occurred in the human lineage. This research has revealed that the microsatellite D14S299 is present in apes and Old World monkeys, and the common structures of the repeat regions are conserved among catarrhide primates. We are now trying the evolutionary analysis by locating the family-, genus- or species-specific differentiations on a given phylogenetic tree.
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