| Project/Area Number |
10670421
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Dokkyo University School of Medicine |
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Principal Investigator |
HIRAO Jun-ichi Dokkyo University, School of Medicine, Lecturer, 医学部, 講師 (80049246)
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| Co-Investigator(Kenkyū-buntansha) |
ENOKIHARA Hideo Dokkyo University, School of Medicine, Associate Professor, 医学部, 助教授 (70049245)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Kawasaki disease / CD4ィイD1+ィエD1CD8ィイD1+ィエD1 T lymphocytes / IL-4 / Anti-IL-4 antibody / IL-2 / Interferon-γ / Th2 / 亢インターロイキン 4抗体 / インターロイキン 2 / インターフェロンーγ / インターロイキン 4 / 細胞内サイトカイン |
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| Research Abstract |
We studied the mechanism of the appearance of a large number of CD4ィイD1+ィエD1 CD8ィイD1+ィエD1 T lymphocytes in the peripheral blood from the patients with Kawasaki Disease. The results were as follows :
1)The peripheral T lymphocytes containing a large number of CD4ィイD1+ィエD1 CD8ィイD1+ィエD1 T lymphocytes were Th2 dominant cytokine-producing pattern, I.e. low dosage of interferon-gamma and interleukin-2 production, and same or high dosage of interleukin-4 production compared with age-matched controls.
2) When the peripheral blood containing a large number of CD4ィイD1+ィエD1 CD8ィイD1+ィエD1 T lymphocytes were cultured with anti-interleukin-4 antibody, CD4ィイD1+ィエD1CD8ィイD1+ィエD1 T lymphocytes decreased, then disappeared. These effects were blocked by recombinant human interleukin-4. Anti-interleukin-2 antibody used for control antibody was unable to change the number of CD4ィイD1+ィエD1CD8ィイD1+ィエD1 T lymphocytes.
3)When the peripheral blood containing a large number of CD4ィイD1+ィエD1CD8ィイD1+ィエD1 T lymphocytes were cultured with interferon-gamma or interleukin-2, CD4ィイD1+ィエD1CD8ィイD1+ィエD1 T lymphocytes were no decreased.
These studies demonstrate that interleukin-4 was the most important cytokine for appearance of a large number of CD4ィイD1+ィエD1CD8ィイD1+ィエD1 T lymphocytes in the peripheral blood from the patients with Kawasaki Disease.
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