| Project/Area Number |
10670422
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Saitama Medical School |
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Principal Investigator |
NAGATA Makoto Saitama Medical School, Assciate professor, 医学部, 助教授 (20211443)
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| Co-Investigator(Kenkyū-buntansha) |
NISHI Yuichi Saitama Medical School, Post Doctral Fellow, 医学部, 助手 (00275893)
TABE Kazuaki Saitama Medical School, Post Doctral Fellow, 医学部, 助手 (60211372)
朱 宰弘 埼玉医科大学, 医学部, 助手 (50245198)
芝崎 正則 埼玉医科大学, 医学部, 助手 (60265395)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Specific immunotherapy / eosinophils / cell adhesion / endothelial cells / trans endothelial migration / mononuclear cells / 免疫療法 / VCAM-1 / 気管支喘息 / 単核球 / サイトカイン / GM-CSF |
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| Research Abstract |
An initial step of eosinophil accumulation to the allergic inflammation site is adhesion of eosinophils to vascular endothelial cells. We examined whether specific immunotherapy modifies this process via the modification of factor(s) generated from mononuclear cells. Peripheral blood mononuclear cells from mite-sensitive atopic asthmatics generated eosinophil adhesion-inducing activity in response to house dust mite. The eosinophil adhesion-inducing activity was significantly attenuated by specific immunotherapy Blocking experiments using anti-cytokine antibodies revealed that GM-CSF is involved in the development of the adhesion-inducing activity We next examined whether immunotherapy modifies the process of eosinophil transendothelial migration. The supernatants of antigen-stimulated mononuclear cells induced eosinophil transmigration across endothelial cells stimulated with IL-4 plus TNF-alpha. We observed that the transmigration activity was actually blocked by an anti-CCR3 antibody, suggesting an involvement of C-C chemokine families. Finally, we observed that the transmigration activity was significantly reduced following specific immunotherapy. These results suggest that immunotherapy attenuates antigen-dependent eosinophil accumulation to allergic inflammation site via the reduction of eosinophil adhesion to and transmigration across endothelial cells
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