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The function analysis of the apoptosis of pancreatic β-cells in autoimmune (Type I)diabetes using microdissection methods

Research Project

Project/Area Number 10670439
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionFukuoka University

Principal Investigator

ANZAI Keizo  Fukuoka Univ., School of Medicine. Assist. Prof., 医学部, 助手 (60258556)

Co-Investigator(Kenkyū-buntansha) SUZUMIYA Junji  Fukuoka Univ., Hospital, Instructor, 病院・講師 (70206556)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsType I diabetes / NOD mice / Apoptosis
Research Abstract

We produced frozen sections from the pancreas of non-obese diabetic (NOD) mice. which used as model animal of autoimmune diabetes (type I). The apoptotic cells were detected within islets of non-diabetic NOD mice by terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) methods (Topics of the internal medicine advance, l998).
The micodissection methods using micromanupulator was adopted in order to analyze mechanism of pathogenesis of the apoptosis, and either pancreatic β cells or infiltrating lymphocytes around and within islets from pancreas section were cut off and were collected. and mRNA was extracted.
In addition, We analyzed gene expression in either islets or lymphocytes, for example IL-4. IFN-γ. FasL, Fas. perforin, granzyme. TNF-receptor family, iNOS and Bcl-2 family associated with apoptosis. by RT-PCR methods. By arranging result at present the contribution preparation is being carried out.
We measured the serum soluble Fas and FasL in the human NK leukemia patient by ELISA methods (British J Haematology. l998). The NOD mice is also being examined the serum soluble Fas and FasL used the similar measuring method.
In order to clarify the difference in population of infiltrated lymphocytes and in the ratio of CD40. CD40L, CD28, CD86 positive cells between diabetic NOD mice and non-diabetic NOD mice (40 wk-old and over), islets with insulitis were immunohistochemically examined for subsets of infiltrated lymphocytes. We found no difference in between the two groups.
In addition, in order to clarify the difference in cytokine and TCR repertoire between B cell deficient NOD mice or B cell deficient streptozocin induced diabetic AKR/J mice and control littermates with B cells. we studied the cytokine and TCR repertoireby infiltrated T cells into islets in both groups by RT-PCR from islets (International Immunology, in press).

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] KEI. KATO, KEIZO. ANZAI: "Elevated serum soluble Fas ligand in natural killer cell proliferative disorders"British J Haematology. 103. 1164-1166 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. KONDO, K. ANZAI: "Analysis of TCR Vβ Usage by Infiltrates of Islets in B cell-Deficient AKR/J Mice with Low Dose-Streptozocin Induced Insulitis and Diabetes"Diabetes. 47 supple. A215 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 近藤しおり、安西慶三: "少量頻回STZ投与B細胞欠損AKRマウスの膵島リンパ球におけるTCR repertoire"Proceedings of the Japanese Society for Immunology. 28 supple. 133 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 岩田 勲、安西慶三: "Bリンパ球欠損NODマウスにおける膵島浸潤リンパ球TCRレパトアの解析"Proceedings of the Japanese Society for Immunology. 28 supple. 133 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 近藤しおり、安西慶三: "STZ誘発自己免疫糖尿病マウスの膵島浸潤リンパ球TCR clonotypeの広がりにおけるB細胞の重要性"Proceedings of the Japanese Society for Immunology. 29 supple. 293 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 安西慶三: "高齢NODマウスにおける糖尿病発症遅延および未発症の機序"J Japan Diabetes Society. 42 supple. 234 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 近藤しおり、安西慶三: "少量頻回 streptozocin(STZ)投与Bリンパ球欠損AKRマウスの膵島炎糖尿病発症抑制機序"J Japan Diabetes Society. 42 supple. 235 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. KONDO, K. ANZAI: "Analysis of TCR Vβ Usage by Infiltrates of Islets in B cell-Deficient AKR/J Mice with Low Dose-Streptozocin Induced Insulitis and Diabetes"International Immunology. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 安西慶三: "内科学進歩のトピックス インスリン依存型糖尿病とアポトーシス"九州大学出版会. 4 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] KEI KATO. KEIZO ANZAI: "Elevated serum soluble Fas ligand in natural killer cell proliferative disorders"British J Haematology. 103. 1164-1166 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] SHIORI KONDO, KEIZO ANZAI: "Analysis of TCR Vβ Usage by Infiltrates of Islets in B cell-Deficient AKR/J Mice with Low Dose-Streptozocin Induced Insulitis and Diabetes"Diabetes. 47 supple. A215 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] SHIORI KONDO, KEIZO ANZAI: "Analysis of TCR Vβ Usage by Infiltrates of Islets in B cell-Deficient AKR/J Mice with Low Dose-Streptozocin Induced Insulitis and Diabetes"International Immunology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary

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Published: 1998-04-01   Modified: 2016-04-21  

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