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HBV DNA integration and expression of HBx messages in HCC

Research Project

Project/Area Number 10670451
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionChiba University

Principal Investigator

YOKOSUKA Osamu  Chiba University, School of Medicine, Lecturer, 医学部, 講師 (90182691)

Co-Investigator(Kenkyū-buntansha) KAWAI Sigenobu  Chiba University, University Hospital, Assistant, 医学部・付属病院, 助手 (10311606)
IMAZEKI Fumio  Chiba University, School of Medicine, Lecturer, 医学部, 講師 (40223325)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsHepatocellular Carcinoma / Hepatitis B virus / Hepatitis C Virus / Integration / PCR / HBx gene / Fusion protein / ALU-HBV / PCR / HBX / fusion蛋白 / Alu-HBV
Research Abstract

Hepatocarcinogenesis was closely associated with hepatitis virus infection in Japan. In the current study, we examined the involvement of HBV in type B and type C hepatocellularcarcinoma in relation to the viral integration and the expression of HBx message.
We analyzed the presence and status of HBV DNA in hepatocelluar carcinoma with Alu-HBV PCR method and conventional HBV PCR method. In type B cases, both integrated and episomal form of HBV were found to be present, whereas in type C cases, episomal form of HBV was mainly found in approximately half of the cases. Thus, involvement of HBV in hepatocarcinogenesis was considered to be different between type B and type C cases.
In type B ceses with HBV DNA integration, various forms of HBx messages were detected including a fusion message of HBV and albumine gene. We are now examining the role of these messages in various signal transduction systems.
In the current study, we have shown the mode of hepatocarcinogesis is different between HCV and HBV infection and that the expression of HBx protein might be important in the carcinogenasis of type B ceses.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Kawai Sigenobu: "Quantitation of hepatitis C Virus by TagMan PCR ; ・・・・・"J. Med Virol. 58. 121-126 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Honda Arata: "Petection of mutations in the enhancer 2/core promoter region of hepatitis B virus with chronic hepatitis B virus infection"J. Med Virol. 57. 337-344 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yokosuka Osamu: "Spontaneous negativation of serum hepatitis C virus RNA is a rare event in type C chronic liver diseases"J. Hepatology. 31. 394-399 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kojima Hiroshige: "Quantitative evaluation of teloncerase activity in small liver tumors"J. Hepatology. 31. 514-520 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seta Toshikatsu: "Emergence of hepatitis B virus with mutations in YMDD motif of DNA polymerase during lamivudine treatment,…"J. Med Virol. 60. 8-16 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tomita Teruyo: "Decrease of wild type and precone mutant DHBV replication during lamivudine treatment in white Peckin dncks・・・・・"J. Hepatology. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Honda A, Yokosuka O, Ehata T, Tagawa M, Imazeki F, Saisho H.: "Detection of mutations the enhancer 2/core promoter region of hepatitis B virus in patients with chronic hepatitis B virus infection : comparison with mutations in precore and core regions in relation to clinical status."J Med Virol. 57. 337-344 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawai S, Yokosuka O, Kanda T, Imazeki F, Maru Y, Saisho H.: "Quantitaion of hepatitis C virus by TaqMan PCR : comparison with HCV amplicor monitor assay."J Med Virol. 58. 121-126 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yokosuka O, kojima H, Imazeki F, Tagawa M, Saisho H, Tamatsukuri S, Omata M.: "Spontaneous negativation of serum hepatitis C virus RNA is a rare event in type C chronic liver diseases : analysis of HCV RNA in 320 patients who were followed for more than 3 years."J Hepatol. 31. 394-399 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kojima H, Yokosuka O, kato N, Shiina S, Imazeki F, Saisho H, Shiratori Y, Omata M.: "Quantitative evaluation of telomerase activity in small liver tumors : analysis of ultrasonography-guided liver biopsy specimens."J Hepatology. 31. 514-520 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seta T, Yokosuka O, Imazeki F, Tagawa M, Ehata T, Saisho H.: "Emergence of hepatitis B virus with mutations in YMDD motif of DNA polymerase during lamivudine treayment in immuno-competent type B hepatitis patients."J Med Virol. 60. 8-16 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tomita T, Yokosuka O, Tagawa M, Saisho H, Tamura S, Fukuda I, Omata M.: "Decrease of wild-type and precore mutant duck hepatitis B virus replication during lamivudine treatment in white Pekin ducks infected with the viruses."J Hepatology. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Honda Arata: "Delection of mutations in the enhancer2/core promoter region of hepatitis B virus with clromic hepatitis B virus infection"J. Med. Virol. 57. 337-344 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kawai Shigenobu: "Quantitation of hepatitis C virus by Taq Man PCR; comparison with HCD amplicor monitor assay"J. Med. Virol. 58. 121-126 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yokosuka Osamu: "Spontaneous negativation of serum hepatitis C virus RNA is a rare event in type C ehronic liuer disenses"J. Hepatol. 31. 394-399 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kojima Hiroshige: "Quantitative evalnation of telomerase activity in small liver tumors"J. Hepatol. 31. 514-520 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Seta Toshikatsu: "Emergence of hepatitis B virus with mutations in YMDD motif of DNA-p during lamivudine treatment・・・・・・"J. Med. Virol. 60. 8-16 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tomita Teruyo: "Decrease of wild-type and precore mutant DHBD replication during lamivudine treatment in white Pekin ducks・・・・・・"J. Hepatol. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kawai S: "Quantitation of hepatitis C viras by Tag Man PCR" J. Medical Virol. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Honda A: "Detection of mutations in the enhancer 2/cone promoter region of HBV …・" J. Medical Virol. 57. 337-344 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yokosuka O: "Spontameous resolution of hepatitis C viremia is a rare event in type C chromic liver disease." J. Hepatol. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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