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肉視鏡的生検材料を用いたヒト小腸粘膜グルコース輸送の検討(グルコース吸収抑制物質の検討)

Research Project

Project/Area Number 10670468
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TSUKADA Hideaki  Kyoto University, Departmemt of Metabol and Clinical Nutrition, Assistant., 医学研究科, 助手 (30207336)

Co-Investigator(Kenkyū-buntansha) FUJITA Jun  Kyoto University, Departmemt of Metabol and Clinical Nutrition, Assistant., 医学研究科, 助手 (00303826)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsDisaccharidase / Ginseng Radix / SGLT / Glucose transporter / GLUT2 / Ussing's method / Triglitazone / Okadaic acid / 糖輸送担体 / Triglitazone / グルコース輸送 / 内視鏡生検
Research Abstract

In this study NaィイD1+ィエD1-dependent glucose transport in human small intestine could be examined using endoscopic biopsy. Comparative study of inhibitory effect on glucose and maltose absorption elucidated that chemicals inhibits disaccharidase such as acarbose or inhibits sodium dependent glucose transporter such as Ginseng Radix (ref,1). Ginseng radix (GR) has been reported to inhibit hyperglycemia. This agent has been mainly used to treat diabetes in clinical practice. It has been reported that the action mechanism of GR involves enhancement of insulin biosynthesis and increased glucose transporter (GLUT-2) expression in the liver. When short-circuit current (Isc) in the human duodenal mucosa was measured before GR administration, Isc was 5.7±3.7uA/cm2 with sugar free Ringer's solution on both the mucosal and serosal sides. Addition of 10mM glucose or maltose to the mucosal side chamber increased Isc 2-10minutes after administration. Subsequently, GR administration inhibited the increase in Isc within 2 minutes in both the glucose-treated group and the maltose-treated group. These results suggests that Ginseng Radix ameliorates hyperglycemia by inhibiting NaィイD1+ィエD1-glucose co-transporter directly. Triglitazone inhibits electrogenic bicarbonate secretion in human small intestine (ref.2). Okadaic acid evokes an increase in water and electrolyte permeability of intestinal epithelium by opening the paracellular pathway (ref.3). Human gastric mucosa obtained by endoscopic biopsy is also useful for electrophysiological study (ref.4).

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Onomura,M., Tsukada,H.: "Effects of ginseng radix on sugar absorption in the small intestine"American Journal of Chinese Medicine. 27Nos.3-4, pp. 347-354 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hosokawa,M., Tsukada,H.: "Triglitazone inhibits bicarbonate secretion in ratand human duodenum"J. Pharmacol. Exp. Ther.. 290(3). 1080-1084 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hosokawa,M., Tsukada,H.: "Effects of okadaic acid on rat colon"Digestive Disease and Science. 43(11). 2526-2535 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuda,K., Tsukada,H.: "Adrenomedullin promotes epithelial restitutim of rat and human qastric mucosa in vitro"Peptides. 20. 127-132 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Onomura, M., Tsukada, H., etal: "Effects of ginseng radix sugar absorption in the small."American Journal of Chinese Medicine. 27(Nos.3-4). 347-354 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hosokawa, M., Tsukada, H, etal: "Tligritazone inhibits bicarbonate secretion in rat and human duodenum"J.Phanmacal.Exp.Ther.. 290(3). 1080-1084 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hosokawa.M., Tsukada, Ketal: "Effects of Okadaic acid onrat colon"Digestive disease and Science. 43(11). 2526-2535 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuda, K, Tsukada, H.: "Adrenomedullin promotes epithelial restitution of rat and human gastric mucosa invitro."Peptides. 20. 127-132 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Onomura,M,Tsukada,H.: "Effects of ginseng radix on sugar absorption in the small intestine"American Journal of Chinese Medicine. 27Nos.3-4pp. 347-354 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hosokawa,M.,Tsukada,H.: "Triglitazone inhibits bicarbonate secretion in rat and human duodenum"J.Pharmacol.Exp.Ther. 290(3). 1080-1084 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hosokawa,M.,Tsukada,H.: "Effects of Okadaic acid on rat colon"Digestive Disease and Science. 43(11). 2526-2535 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fukuda,K.,Tsukada,H.: "Adrenomedullin promotes epithelial restitution of rat and human gastric mucosa in vitro"Peptides. 20. 127-132 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hosokawa M,et al.: "Effects of okadaic acid on rat colon" Digestive Disease and Science. 43(11). 2526-2535 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fukuda K,et al.: "Effects of Adrenomedullin on Ion Transport and Muscle Contraction in Rat Distal Colon" Peptides. 19(6). 1043-1047 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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