A standardized rat model of Helicobacter pylori infection and a development of experimental gastritis and ulcer
| Project/Area Number |
10670493
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Nagoya City University |
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Principal Investigator |
SENO Kyoji Nagoya City University Medical School, Research Associate, 医学部, 助手 (30295589)
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| Co-Investigator(Kenkyū-buntansha) |
JOH Takashi Nagoya City University Medical School, Associate Professor, 医学部, 助教授 (30231369)
AGUI Takashi Nagoya City University Medical School, Associate Professor, 医学部, 助教授 (00212457)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | animal model / Helicobacter pylori / mucosal injury / H.pylori |
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| Research Abstract |
Background ＆ Aim : Currently available Helicobacter pylori (H. pylori) models show variable and, in some instances, poor colonizing ability to act as a standard for animal studies, we tried to classify a H. pylori-infectable model rat.
Methods : Rats were administered at three times with live H. pylori on the 28 - 35 days old. More 28 days later, gastric juice of each rat was collected and it was estimated that H. pylori was infected or not. Male and female H. pylori-infected rats mated and bared babies. The baby-rats were administered with live H. pylori in the same manner.
Results : The thirteenth generation was born. The rate of infection of H. pylori was increased significantly from 25.0 % (for the first generation) to 64.2 % (for the 13th generation).
Conclusion: H. pylori-infectable model rat was developed, but pure lineage was still not standardized. The method of measuring the mucosal damage of stomach was developed and published. The method to detect the specific antibodies against H. pylori-surface antigen was developed and published.
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Report
(5 results)
Research Products
(7 results)
