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Biological modulation of the epithelial-mesenchymal interaction in gut wound repair process.

Research Project

Project/Area Number 10670503
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionAKITA UNIVERSITY (1999)
Juntendo University (1998)

Principal Investigator

WATANABE Sumio  School of Medicine, Akita University, Professor, 医学部, 教授 (20138225)

Co-Investigator(Kenkyū-buntansha) OTAKA Michiro  School of Medicine, Akita University, Assistant, 医学部, 助手 (30250872)
MIYAZAKI Akihisa  School of Medicine, Juntendo University, Associate Professor, 医学部, 助教授 (20200149)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsgastric ulcer / growth factor / cytokine / migration / differentiation / Helicobacter pylori / HSP / stress / 損傷修復 / 細胞分裂 / ヘリコバクターピロリ / 胃粘膜損傷 / 消化性潰瘍 / 細胞培養 / 線維芽細胞 / 血管内皮細胞 / インスリン様増殖因子 / 細胞外基質
Research Abstract

It has been generally accepted that the epithelial-mesenchymal interaction plays an important role in gut would repair process. In this project, we investigated the detailed mechanism of action of several growth factors, cytokines, ECM, toxic agents, trace minerals and physical stress in gut would healing using in vivo and in vitro would healing model. As expected, each growth factor has its own target cells and some growth factors show their effects by paracrine and autocrine mechanism. Among these growth factors, insulin-like growth factor 1 played a key role in gastric wound healing in vivo and in vitro. In this mechanism zinc stimulated the production of insulin-like growth factor 1 from fibroblasts and endothelial cells and induced insulin-like growth factor 1 affected to gastric epithelial cell migration and proliferation. In another series of experiments, we showed that heat shock protein 72 has cytoprotective effect in the stomach and pre-induction of heat shock protein 72 prevented gastric mucosal damage in the several experimental model with rats. In the experiment using strain stress in vitro, heat shock protein 72 was found in decreased significantly in the gastric epithelial cells and strain stress also inhibited gastric epithelial migration and proliferation resulting in the delay of wound healing. In the clinical situation, we found the inhibition of the heat shock protein expression in the Helicobacter pylori infected gastric mucosa. Therefore heat shock protein as well as growth factors may play an important role in the integrity of gastric mucosal damage and wound healing.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] S Watanabe,XE Wang,M Hirose,T Yoneta,K Seto,N Sato,et al: "Insulin like growth factor-1 plays a Role in gastric wound healing : Evidence using a zinc derivative, polaprezinc, and an in vitro rabbit wound repair mode"Aliment Pharmacol Ther. 12. 1131-1138 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N Sato,S Watanabe,Xe Wang. T Osada S Yamashina H Miwa,et al.: "A histamine H2 Receptor antagonist, FRG-8813, prevented the delay of wound repair induced by hydrogen peroxide in a rabbit gastric epithelial cell system"J Gastroenterol Hepatol. 13. S209-S213 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Miwa,T Murai,R Ohkura, A Nagahara,T Ogihara,S Watanabe,N Sato: "13C urea breath test for cure of Helicobacter pylori infection at 1 month after treatment"J Clin Gastroenterol. 27. S150-S153 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T Itatsu,H Miwa,R Ohkura,R Iwazaki,S Watanabe,S Hirai,M Otaka,N Sato,et al.: "Primary gastric T-cell lymphoma accompanied by HTLV-1, HBV and H. pylori infection"Dig Dis Sci. 44. 1823-1836 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T Osada,K Iijima,H Tanaka,M Hirose,J Yamamoto,S Watanabe: "Effect of temparature and mechanical strain on gastric epithelial cell line GSMO6 wound restoration in vitro"J Gastroenterol hepatol. 14. 489-494 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S Watanabe, XE Wang, M Hirose, T Kivilioto, T Osada, H Miwa, H Oide, T Kitamura, T Yoneta, K Seto, N Sato: "Insulin like growth factor-1 plays a Role in gastric would healing : Evidence using a zinc derivative, polaprezinc, and an in vitro rabbit wound repair model."Aliment Pharmacol Ther. 12. 1131-1138 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N Sato, S Watanabe, Xe Wang, T Osada, H Tanaka, T Itatsu, R Miyata, K Watanabe, K Sato, M Nakajima, S Yamashina, H Miwa: "A histamine H2 Receptor antagonist, FRG-8813, prevented the delay of wound repair induced by hydrogen peroxide in a rabbit gastric epithelial cell system."J Gastoenterol Hepatol. 13. s209-s213 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Miwa, T Murai, R Ohkura, A Nagahara, T Ogihara, S Watanabe, N Sato: "13C urea breath test for cure of Helicobacter pylori infection at 1 month after treatment."J Clin Gastroenterol. 27s1. s150-153 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T Itatsu, H Miwa, R Ohkura, R Iwazaki, H Oide, T Murai, M Nakajima, S Watanabe, S Hirai, M Otaka, N Sato: "Primary gastric T-cell lymphoma accompanied by HTLV-1, HBV and H. pylori infection."Dig Dis Sci. 44. 1823-1836 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T Osada, K Iijima, H Tanaka, M Hirose, J Yamamoto, S Watanabe: "Effect of temperature and mechanical strain on gastric epithelial cell line GSM06 wound restoration in vitro."J Gastroenterol hepatol. 14. 489-494 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Jin M, Otaka M, Watanabe S, et al.: "Association of 72-KDa Heat Shock Protein Expression with Adaptation to Aspirin in Rat Gastric Mucosa"Digestive Diseases and Sciences. 44・7. 1401-1407 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Itatsu T, Miwa H, Watanabe S, Sato N, et al.: "Primary gastric T-cell lymphoma accompanied by HTL V-I, HBV and H pylori infection"Digest Dis Sci. 44・9. 1823-1836 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ymamoto J, Watanabe S, Hirose M, Sato N, et al.: "Role of most cell as a trigger of inflammation in Helicobacter pylori infection"J Physiol Pharacol. 50・1. 17-23 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osada T, Iijima K, Watanabe S, et al.: "Effect of temperature and mechanical strain on gastric epithelial cell line GSM06 wound restoration in vitro"J Gastroenterol Hepatol. 14・5. 489-494 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sato N,et al.: "The novel histamine H2 receptor antagonist,FRG-8813,prevents delay of wound repair induced by hydogen peroxide in a rabbit gastric epithelial cell system." Journal of Gastroenterology and Hepatology. 13(Supple). 209-213 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe S,et al.: "Insulin-like growth factor I plays a role in gastric wound healing: evidence using a zinc derivative,polaprezinc,and an in vitro rabbit wound repair model." Alimentary Pharmacology and Therapeutics. 12. 1131-1138 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe S,et al.: "Rebamipide prevented delay of wound repair induced by hydrogen peroxide and suppressed apoptosis of gastric epithelial cells in vitro." Digestive Diseases and Sciences. 43(9). 107S-112S (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe S,et al.: "Effects of teprenone on gastric epithelial restoration in a rabbit cultured cell model." Journal of Gastroenterology. 33. 153-159 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe S,et al.: "A neurotorophic pyrimidine compound,MS-818,enhances EGF-induced restoration of gastric epithelial wounds in vitro." Journal of Clinical Gastroenterology. 27(Suppl.1). S105-S109 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Oide H,et al.: "The effect of interferon γ on hepatic haemodynamics." Journal of Gastroenterology and Hepatology. 13(10). 975-976 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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