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Analysis of ICAM-1-independent intestinal epithelial cell adhesion to neutrophils

Research Project

Project/Area Number 10670504
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionJuntendo University

Principal Investigator

SOMEYA Akimasa  Juntendo University, School of Medicine, Assistant Professor, 医学部, 講師 (90167479)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsIntestinal epithelial cell / Neutrophil / Inflammation / Adhession molecule / Inflammatory mediator / Cytokine / TNF-α / Histamine
Research Abstract

Neutrophils play an important role in intestinal inflammation by interacting with intestinal epithelial cells. In this study, we evaluated neutrophil adhesion to intestinal epithelial cells using intestinal epithelial cell line HT29 stimulated with tumor necrosis factor a (TNF-α) and histamine.
(1) The TNF-α and histamine stimulation markedly increased neutrophil adhesion. The increased adhesion was inhibited by anti-CD11b and anti-CD18 antibodies, but not by anti-CD11a and anti-CD54 (ICAM- 1) antibodies.
(2) Interestingly, flow cytometric analysis revealed that ICAM-1 expression on HT29 cells was not changed by TNF-αand histamine stimulation. These observations indicate that exposure of HT29 cells to TNF-αand histamine induces CD11b/CD18 (Mac-1)-dependent but CD11a/CD18 (LFA-1)-independent neutrophil adhesion to intestinal epithelial cells, and ICAM-1 is not likely involved in the interactions.
(3) Moreover, the increased adhesion was inhibited by proteinase K treatment but not cyclohexi … More mide treatment of HT29 cells, suggesting that epithelial cell ligand(s) for neutrophils is likely protein molecule(s) that is exposed on the cell by stimulation independent of protein synthesis.
(4) Even when HT29 cells were treated with endoglycosidase H, sialidase or tunicamycin, neutrophil adhesion to TNF-α- or histamine-stimulated HT29 cells was not affected, suggesting that sugar chains may noy be involved in CD11b/CD18-mediated neutrophil adhesion to HT29 cells.
(5) Treatment of HT29 cells with phosphatidylinositol-specific phospholipase C which cleaves glycosylphosphatidyl inositol (GPI)-anchored protein, did not affect neutrophil adhesion to TNF-α- or histamine-stimulated HT29 cells. In addition, neither RGD peptide nor heparinase treatment of HT29 cells affected neutrophil adhesion to stimulated HT29 cells. These results suggest that GPI-anchored protein, heparin-like glycosaminoglycans and extracellular matrix protein containing RGD sequences are unlikely involved in neutrophil-HT29 cell interactions. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Miyata R. et al: "Short exposure of intestinal epithelial cells to TNF-α and histamine induces Mac-1-mediated neutrophil adhesion independent of protein synthesis"J. Leukoc. Biol.. 66. 437-446 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyata R. et al: "Exposure of intestinal epithelial cell TH29 to bile acids and ammonia enhances Mac-1-mediated neutrophil adhesion"Inflamm. Res.. 48. 265-273 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyata R. et al.: "Short exposure of intestinal epithelial cells to TNF-α and histamine induces Mac-1-mediated neutrophil adhesion independent of protein synthesis"J. Leukoc. Biol.. 66. 437-446 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyata R. et al.: "Exposure of intestinal epithelial cell HT29 to bile acids and ammonia enhances Mac-1-mediated neutrophil adhesion"Inflamm. Res.. 48. 265-273 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyata R. et al.: "Short exposure of intestinal epithelial cells to TNF-α and histamine induces Mac-1-mediated neutrophil adhesion independent of protein synthesis"J. Leukoc. Biol.. 66. 437-446

    • Related Report
      1999 Annual Research Report
  • [Publications] Miyata R. et al.: "Exposure of intestinal epithelial cell HT29 to bile acids and ammonia enhances Mac-1-mediated neutrophil adhesion."Inflamm. Res.. 48. 265-273

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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