|Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
In this study, cDNA representation difference analysis (RDA) was used to identify candidate genes selectively expressed in liver metastasis of colon cancer which are not expressed in primary colon cancer. In RDA with the primary cancer as "tester" and an excess amount of the metastasis as "drivers", no discrete bands were seen. In RDA with the metastasis as "tester" and the primary cancer as "drivers", 8 candidate genes were found. Of 8 candidate genes, four were found to be identical to Reg 1α, GW112, Annexin-1 and Bax by DNA sequencing. Two were found to be the genes submitted to GenBank (one was the gene cloned in cosmid L219 and the other was the gene on chromosome 16p13), although the function of these genes were unkown. The other two clones were not identical to any of the genes submitted to GenBank. Of these, we further examined for Reg 1α, Annexin-1 and Bax functions of which are reported, and for GW112, which is the dominant species in RDA.During further examination of these genes, we found that the expression of these genes were not restricted to metastasis, but closely related to the inflammation of colon. We showed the relation between Bax gene expression and inflammation of the colon and the relation between leg 1α, Annexin-1 and GW112 gene expression and inflammation of the colon in detail in the report.