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The Biological Functions of the SART-1 Family in Differentiation and Carcinogenicity of Adenocarcinomas

Research Project

Project/Area Number 10670521
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKurume University

Principal Investigator

SHICHIJO Shigeki  Kurume Univ., Sch. Med., Dep. Immunol., Associate Prof., 医学部, 助教授 (30080592)

Co-Investigator(Kenkyū-buntansha) ITOH Kyogo  Kurume Univ., Sch. Med., Dep. Immunol., Prof., 医学部, 教授 (50125499)
IMAI Yasuhisa  Kurume Univ., Sch. Med., Department of lmmunol., Assistant, 医学部, 助手 (90268847)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsTumor rejection antigen / DNA-binding protein / Cell cycle / Apoptosis / Two-hybrid / ファーウエスタン / 遺伝子ファミリー / ゲノムDNA / SART 1 / 核内蛋白 / ロイシン・ジッパー・モチーフ / ファー・ウェスタン
Research Abstract

Molecules involved in regulation of cellular proliferation at the G2/M phase have not yet been fully identified. This study investigated the involvement of a recently identified SART-1ィイD2800ィエD2. Protein demonstrated an ability to bind to DNA, and accumulated in the nucleus when the cells were arrested at G2/M phase.
(l) The localization of SART-1 protein was suggested to be in nucleus of eukaryote cells including monkey kidney EBV-transformed Cos 7 cell and human esophageal cancer TE9 cell when the fusion gene with GFP was transfected and observed by conforcal laser microscopy.
(2) SART-1ィイD2800ィエD2 protein is suggested to be a DNA-binding protein by the affinity chromatography of cell lysates of the 293T cells transfected with the HAN/SART-1ィイD2800ィエD2 gene.
(3) Transfection of SART-1 gene induced the G2/M-arrest and suppressed the cell growth of all the cell tested. Furthermore, DNA ladder formation was observed in the SART-1 transfected COS-7 and human cancer cells. The transfection of SART-1 up-regulated the nuclear expression of cyclin B and cdc2, well known components of maturation-promoting factor.
(4) Several candidate genes which encode proteins that had interacted with SART-1 protein were isolated by two-hybrid system.
(5) Three different SART-1 probe protein (N-terminal, middle and C-terminal regions) for far-western blot analysis of SART-1 binding protein candidate obtained by two hybrid system were prepared.
(6) We found that murine SART-1 genomic DNA suggested to be consist of 20 exsons. The sequence of the gene will submit to GenBank.
(7) SART-1 gene was suggested to be expressed in early development of mouse embryo (l4 days) by northern blot analysis.
(8) Several family genes of SART-1 were suggested by northern blot analysis. One of the gene with 3.3kb consist of approximately l488 bp of 5'-region was identical to SART-1 and encoded a protein with 483 amino acid.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Shigeki Shichijo: "A gene encoding antigenic peptides of human squamous cell carcinoma recognized by cytotoxic Tlymphocytes"Journal of Experimental Medicine. 187. 277-278 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mayumi Kawamoto: "Expression of the SART-1 tumor-rejection antigen in breast cancer"International Journal of Cancer. 80. 64-67 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Megumi Kikuchi: "Identification of a SART-1-derived peptide capable of inducing HLA-A24-restricted and tumor-specific cytotoxic lymphocytes"International Jounal of Cancer. 81. 459-466 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Imaizumi: "Expression of the tumor-rejection antigen SARTI in brain tumors"International Journal of Cancer. 83. 760-764 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Damu Yang: "Identification of a gene coding for a protein possessing shared tumor epitopes capable of inducing HLA-A24-restricted cytotoxic Tlymphocytes in cancer patients"Cancer Research. 59. 4056-4063 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Masanobu Nakao: "Identification of a gene coding for a new squamous cell carcinoma antigen recognized by the CTL"Journal of Immunology. 164. 2565-2574 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigeki Shichijo: "Gann Monograph on Cancer Reseach"Japan Scientific Societies Press. 9 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kyogo Itoh: "Cell Therapy"Springer-Verlag Tokyo (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shichijo S, Nakao M, Imai Y,Takasu H, Kawamoto M, Niiya F, Yang D, Toh Y, Yamana H and ltoh K: "A gene encoding antigenic peptides of human squamous cell carcinoma recognized by cytotoxic T lymphocytes."J. Exp. Med.. 187. 277-288 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Gotoh M, Shichijo S, Hoshino T, lmai Y, Imaizumi T, lnoue Y, Takasu H, Yamaoka T and Itho K: "Sequence analysis of genes encoding rodent homologues of the human tumor-rejection antigen SART-1."Jpn. J. CancerRes. 89. 849-854 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matumoto H, Kwano K, Nishida T, Sakamoto M, and Itoh K: "Expression of the SART-1 antigens in uterine cancers."Jpn. J. CancerRes. 89AA. 1292-1295 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shichiko S, Imai Y, Nakao M, Hayashi A, Yamana H, and Itoh K: "human Squamous cell carcinoma antigen gene, SART-1."Cell Technology. (Japanese) 17. 1216-1222 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawamoto M, Shichijo S, Imai Y, lmaizumi T, Koga T, Yanaga H, and Itoh K: "Expression of the SART-1 tumor-rejection antigen in breast cancer."Int. J. Cancer. 80. 64-67 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kikuchi M, Nakao M, Inoue Y, Matsunaga K, Shichij S, Yamana H, and Itoh K: "Identification of a SART-1-derived peptide capable of inducing HLA-A24-restricted and tumor-specific cytotoxic lymphocytes."Int. J. Cancer. 81. 459-466 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] lmaizumi T, Kuramoto T, Matsunaga M, Shichijo S, Yutani S, Oizumi K and Itoh K: "Expression of the tumor-rejection antigen SART-1 in brain tumors."Int. J. Cancer. 83. 760-764 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yang D, Nakao M, Shichijo S, Sasatomi T, Takasu H, Matsumoto H, Mori K, Hayashi A, Yamana H, Shirouzu K, and Itoh K: "Identification of a gene coding for a protein possessing shared tumor epitopes capable of inducing HLA-A24-restricted cytotoxic T lymphocytes in cancer patients."Cancer Res. 59. 4056-4063 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakao M, Shichijo S, Imaizumi T, Inoue Y, Matsunaga K, Yamada A, Kikuchi M, Tsuda N, Ohta K, Takamori S, Yamana H, Fujita H and Itoh K: "Identification of a gene coding for a new squamous cell carcinoma antigen recognized by the CTL."J. Immunol.. 164. 2565-2574 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shichijo S, Nakao M, Imai Y, Takasu H, Hayshi A, Toh U, Yamana H and ltoh K: "A gene encoding squamous cell carcinoma antigens recognized by cytotoxic T lymphocyte. In : Recent Advances of Human Tumor Immunology and Immuno-therapy. (Gann Monograph on Cancer Research, No. 48). (eds.) Kikuchi K, and Sato T"Japan Scientific Societies Press, Tokyo. 43-51 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Itoh K, Shichijo S, lnoue Y, Hayashi A, Toh U, Yamana H: "SART-1 gene encoding squamous cell carcinoma antigen recognized by cytotoxic T lymphocytes. In : Cell Therapy. (eds) Ikeda Y, and Kawakami Y"Springer-Verlag Tokyo, (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigeki Shichijo: "A gene encoding antigenic peptides of human squamous cell carcinoma recognized by cytotoxic T lymphocytes."Journal of Experimental Medicine. 187. 277-278 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mayumi Kawamoto: "Expression of the SART-1 tumor-rejection antigen in breast cancer."International Journal of Cancer. 80. 64-67 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Megumi Kikuchi: "Identification of a SART-1-derived peptide capable of inducing HLA-A24-restricted and tumor-specific cytotoxic lymphocytes."International Journal of Cancer. 81. 459-466 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Imaizumi: "Expression of the tumor-rejection antigen SART1 in brain tumors."International Journal of Cancer. 83. 760-764 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Damu Yang: "Identification of a gene coding for a protein possessing shared tumor epitopes capable of inducing HLA-A24-restricted cytotoxic Tlymphocytes in cancer patients."Cancer Research. 59. 4056-4063 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masanobu Nakao: "Identification of a gene coding for a new squamous cell carcinoma antigen recognized by theCTL."Journal of Immunology. 164. 2565-2574 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shigeki Shichijo: "Gann Monograph of Cancer Reseach"Japan Scientific Societies Press. 9 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kyogo Itoh: "Cell Therapy"Springer-Verlag Tokyo (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shichijo S.,et al.: "A gene encoding antigenic peptides of human squamous cell carcinoma recognized by cytotoxic T lymphocytes." J.Exp.Med.,. 187. 277-288 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Gotoh M.,Shichijo S.,et al.: "Sequence analysis of genes encoding rodent homologues of the human tumor-rejection antigen SART-1." Jpn.J.Cancer Res.,. 89. 845-854 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto H.,Shichijo S.,et al.: "Expression of the SART-1 antigens in uterine cancers." Jpn.J.Cancer Res.,. 89. 1292-1295 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kawamoto M.,Shichijo S.,et al.: "Expression of the SART-1 tumor-rejection antigen in breast cancer." Int.J.Cancer. 80. 64-67 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kikuchi M.,Shichijo S.,et al.: "Identification of a SART-1-derived peptide capable of inducing HLA-A24-restricted and tumor-specific cytotoxie lymphocytes." Int.J.Cancer. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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