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Elucidation of mechanism of leukocyte migration using an optical

Research Project

Project/Area Number 10670529
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

YANAI Masaru  Tohoku University Hospital, Lecturer, 医学部・附属病院, 講師 (00210287)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordscytoskeleton / rheology / biomechanics / pseudopodia / 仮足 / 細胞骨格 / 粘弾性 / 細胞内圧
Research Abstract

To investigate the mechanisms underlying pseudopod protrusion in locomoting neutrophils, we measured the intracellular stiffness and viscosity in the leading region (L), main body (B), and trailing region (T) from displacements of oscillating intracellular granules driven with an optical trap. Experiments were done in control conditions, and following treatment with cytochalasin D or nocodazole. We found (1) in B and T, the granules divided into a fixed population (too stiff to measure), and a free population (easily oscillated) (fixed fraction 65%, free fraction 35%). By contrast, the fixed fraction in L was <5%. (2) In B and T, there was no difference in stiffness or viscosity, but both were sharply lower in L (respectively 20 fold and 5 fold). (3) Neither cytochalasin D nor nocodazole caused a decrease in stiffness, but both treatments markedly reduced the fixed fraction in B and T to <20% and <40% respectively. These observations suggest a discrete lattice structure in B and T, and that the developing pseudopod has a core that is more fluid-like, in the sense of a much lower viscosity and an almost total loss of stiffness. This is consistent with the contraction/solation hypothesis of pseudopodial formation.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yanai M. et al.: "Intracellular elasticity and viscosity in the body, leading, and trailing regions of locomoting neutrophils"Am.J. Physiol.. 277. C432-C440 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakayama M. et al.: "Production and secretion of adrenomedullin in cultured human alveolar macrophages"Peptides.. 1123-1125 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sekizawa K. et al.: "Amantadine and pneumonia in elderly stroke patients"Lancet.. 353. 2157 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakajoh K. et al: "Longevity may decrease medical costs"J. Am. Geriatr . Soc .. 47. 1161-1162 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sekizawa K. et al.: "Cor pulmonale and tumour cell microemboli"Lancet. 352. 822 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sasaki H. et al .: "Review : Effects of air pollution and smoking on chronic obstructive pulmonary disease and bronchial asthma"Tohoku J. Exp . Med.. 186. 151-167 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yanai M. et al.: "Deposition and clearance of inhaled ^<18>FDG powder in patients with chronic obstructive pulmonary disease"Eue . Respir. J.. 11. 1342-1348 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yanai M. et al.: "Intracellular elasticity and viscosity in the body, leading, and trailing regions of locomoting neutrophils"Am. J. Physiol.. 277. C432-C440 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakayama M. et al.: "Production and secretion of adrenomedullin in cultured human alveolar macrophages"Peptides.. 1123-1125 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sekizawa K. et al.: "Amantadine and pneumonia in elderly stroke patients"Lancet.. 353. 2157 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakajoh K. et al.: "Longevity may decrease medical costs"J. Am. Geriatr. Soc.. 47. 1161-1162 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sekizawa K. et al.: "Cor pulmonale and tumour cell microemboli"Lancet.. 352. 822 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sasaki H. et al.: "Review : Effects of air pollution and smoking on chronic obstructive pulmonary disease and bronchial asthma"Tohoku J. Exp. Med.. 186. 151-167 (19**)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yanai M. et al.: "Deposition and clearance of inhaled ィイD118ィエD1FDG powder in patients with chronic obstructive pulmonary disease"Eue. Respir. J.. 11. 1342-1348 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yanai M.et al.: "Intracellular elasticity and viscosity in the body, leading and trailing regions of locumoting neutrophils"Am. J. Physicol. 277. C432-C440 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakayama M.et al.: "Production and secretion of adrenomedullin in cultured human alveolar macrophages"Peptides. 20. 1123-1125 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sekizawa K.et al.: "Amantadine and pneumonia in elderly stroke patients"Lancet. 353. 2157 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakajoh K.et al.: "Longevity may decrease medical costs"J Am Geriat Soc.. 47. 1161-1162 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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