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Role of airway epithelial cells in the accumulation and activation of T cells in allergic airway inflammation

Research Project

Project/Area Number 10670533
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionThe University of Tokyo

Principal Investigator

TAKIZAWA Hajime  The Univ. Tokyo, Dpt. Med. Phys. Therapy, Lecturer, 医学部・附属病院, 講師 (80171578)

Co-Investigator(Kenkyū-buntansha) SATO Makoto  The Univ. Tokyo, Dpt. Med. Phys. Therapy, Assist Prof. of Med., 医学部・附属病院, 医員
ISHII Akira  The Univ. Tokyo, Dpt. Med. Phys. Therapy, Assist Prof. of Med., 医学部・附属病院, 助手 (30272553)
SHOJI Shunsuke  The Univ. Tokyo, Dpt. Med. Phys. Therapy, Assist Prof. of Med., 医学部・附属病院, 助手 (10171018)
KOHYAMA Tadashi  The Univ. Tokyo, Dpt. Med. Phys. Therapy, Assist Prof. of Med., 医学部・附属病院, 医員
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsAllergic airway inflammation / Bronchial asthma / Chemokine gene expression / Airway epithelial cell / T cell / Chemotactic factor / Adhesion molecule / co-stimulatory molecule / アレルギー性気道炎症 / ケモカイン / RANTES / ICAM-1
Research Abstract

Airway epithelial cells are known to play an integral role in the airway responses thorough the production of cytokines/chemokines important in the pathogenesis of bronchial asthma. We could demonstrated that : 1) human airway epithelial cells had potentials to express and release RANTES and TARC , both being chemotactic for T lymphocytes. 2) They expressed adhesion molecules and co-stimulatory molecules such as ICAM-1 (CD54). B7-1 CCD80) and B7-2 (CD86). When T lymphocytes were co-incubated with airway epithelial cells, they were stimulated to release IL-4 and IL-13, which are important Th2-type cytokines. Therefore, airway epithelial cells can regulate not only cell migration and attachment but also activation of T cells. 3) In murine models of allergic asthma, there was an intense expression of TARC in the airway epithelium by immunohistochemistry. Above observations strongly suggested that airway epithelial cells play an important role in the regulation of T lymphocyte functions in allergic airway inflammation found in asthma.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report

Research Products

(32 results)

All Other

All Publications (32 results)

  • [Publications] Takizawa H.: "Airway epithelial cells as regulators of airway inflammation"International Journal of Molecular Medicine. 1. 367-378 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Kawasaki, H. Takizawa, T. Ohtoshi, N. Takeuchi, T. Kohyama, H. Nakamura, T. Kasama, K. Kobayashi, K. Nakahara, Y. Morita, K. Yamamoto: "Roxithromgcin Inhibits Cytokin Production and Neutrophile Attachment with Human Bronchial Epithelial Cells in vitro"Antimicrob. Agents Chemother.. 42. 1499-1502 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Kobayashi, T. Niitsuma, T. Hayashi, M. Tanaka, H. Takizawa: "Interferon-γ inhibits the growth of human bronchial epithelial Cells independently of transforming growth factor - B-1 and nitric oxide (NO)"Biochem. Biophys. Res. Commun.. 224. 126-130 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Ohtoshi, H. Takizawa, H. Okazaki, S. Kawasaki, N. Takeuchi, K. Ohta, K. Ito: "Diesel exhaust particles (DEP) stimulate human airway epithelial Cells to produce cytokines relevant to airway inflammation in vitro"J. Allergy Clin. Immunol.. 101. 778-785 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto K., Hashimoto S., Gon Y., Nakamura T., Takizawa H., Horie T.: "Nacethylcystein inhibits IL-1α-induced IL-8 secretion by bronchial epithelial cells"Respir. Med.. 92. 512-515 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kusunoki S., Takizawa H., Kawasaki I.: "Infection by mycoplasma pneumoniae induces serum antibody against gal-C"Muscle & Nerve. 19. 257-258 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 滝沢 始: "Wegener肉芽腫症・血管炎・外来診療のすべて"メジカルビュー社. 2 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 滝沢 始: "アレルギー性気管支肺アスペルギルス症 私の第一選択薬"治療増刊号・南山堂. 2 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tadashi Kohyama, Hajime Takizawa, Shin Kawasaki, Norihisa Akiyama, Makoto Sato and Koji Ito: "Fourteen-member macrolides inhibit IL-8 release by human eosinophils from atopic donors."Antimicrob. Agents Chemother.. 43. 907-911 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hajime Takizawa, Takayuki Ohtoshi, Shin Kawasaki Tadashi Kohyama, Masashi Desaki, Tsuyoshi Kasama Kazuo Kobayashi, Kazuhiko Nakahara, Kazuhiko Yamamoto, Kouji Matsushima and Shoji Kudoh: "Diesel, exhaust particles ( DEP ) induce nuclear tactor-kappa B ( NFκB ) activation in human bronchial epithelial, cells in vitro: importance In cytokine transcripton."J. Immunol.. 162. 4705-4711 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Kohyama, H. Takizawa, S. Kawasaki, N. Akiyama, M. Sato, K. Ito, K. Yamamoto: "A potent immunosuppressant FK506 inhibits IL-8 expression in human eosinophils."Mol. Cell Biol. Res. Commun.. 1. 72-77 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Masato Kobayashi, Tomoyuki Niitsuma, Torhu Hayashi, Mitsuru Tanaka, Hajime Takizawa: "Interferon-γ inhibits the growth of human bronchial epithelial cells independently of transforming, growth factor-β-1 and nitric oxide(NO)."Blochem. Blophys. Res. Commun.. 244. 126-130 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto K. Hashimoto S. Gon Y, Nakayama T. Takizawa H. Hone T.: "N-acethylcysteine inhibits IL-1 α-induced IL-8 secretion by bronchial epithellal cells."Respir. Med.. 92. 512-515 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kusunold S, Takizawa H, Kanazawa I: "Infection by mycopiasma pneumoniae induces serum antibody against gal-c."Muscle & Nerve. 19. 257-258 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hajime Takizawa, Masashi Desaki, Takayuki Ohtoshi, Shin Kawasaki, Tadashi Kohyama, Makoto Sato, Jun Nakajima, M. Yanagisawa, Koji Ito.: "Erythromycin and clarithromycin attenuate cytokine-induced endothelin-1 expression human bronchial epithelial cells."Eur. Respir. J.. 12. 57-63 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takizawa H: "Cytokines/chemokines and adhesion molecules in local in inflammatory responses of the lung."Drug News Perspect. 11. 611-619 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takizawa H.: "Airway epithelial cells as regulators of airway inflammation"International Journal of Molecular Medicine. 1. 367-378 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. Kawasaki, H. Takizawa, T. Ohtoshi, N. Takeuchi, T. Kohyama, H. Nakamura, T. Kasama, K. Kobayashi, K. Nakahara, Y. Morita, K. Yamamoto: "Roxithromycin Inhibits Cytokine Production and Neutrophile Attochment with Human Bronchial Epithelial cells in vitro"Antimicrob. Agents Chemother.. 42. 1499-1502 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Kobayashi, T.Niitsuma, T. Hayashi, M. Tanaka, H. Takizawa: "Interferon-γ inhibits the growth of human bronchial epithelial cells independently of transforming growth factor-B-1 and nitric oxide (No)"Biochem. Biophys. Res. Comman.. 224. 126-130 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Ohtoshi, H. Takizawa, H. Okazaki, S. Kawasaki, N. Takeuchi, K. Ohta, K.Ito: "Diesel exhaust particles(DEP) stimulate human airway epithelial cells to produce cytokines relevant to airway inflammation in vitro"J. Allergy Clin. Immunol.. 101. 778-785 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumoto K, Hashimoto S, Gon Y, Nakayama T, Takizawa H, Horie T,: "Nacethylcysteine inhibits IL-1α-induced IL-8 securetion by bronchial epithelial cells"Respir. Med.. 92. 512-515 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kusunoki S, Takizawa H, Kawasaki I: "Infection by mycoplasma pneumoniae induces serum antibody against gal-c"Muscle & Nerve. 19. 257-258 (1996)

    • Related Report
      1999 Annual Research Report
  • [Publications] 滝沢 始: "Wegener肉芽腫症・血管炎 外来診療のすべて"メジカルビュー社. 2 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 滝沢 始: "アレルギー性気管支肺アスペルギス症.私の第一選択薬"治療増刊号・南山堂. 2 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakajima J et al.: "Effect of cryopreservation on the allogenicity of an airway epithelial cell line." Transplant.Proc.30. 3366-3367 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin Kawasaki et al.: "Roxithromycin Inhibits Cytokine Production and Neutrophil Attachment with Human Bronchial Epithelial Cells in vitro.Antimicrob." Antimicrob.Agents Chemother.42. 1499-1502 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masato Kobayashi et al.: "Interferon-γ innibits the growth of human bronchial epithelial cells independently of transforming growth factor-β-1 and nitric oxide(NO)." Biochem.Biophys.Res.Commun.244. 126-130 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takayuki Ohtoshi et al.: "Diesel exhaust particles(DEP)stimulate human airway epithelial cells to produce cytokines relevent to airway inflammation in vitro." J.Allergy Clin.Immunol.101. 778-785 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto K et al.: "N-acethylcysteine inhibits IL-1α-induced IL-8 secretion by bronchial epithelial cells." Respir.Med.92. 512-515 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hajime Takizawa et al.: "Erythromycin and clarithromycin attenuate cytokine-induced endothelin-1 expression in human bronchial epithelial cells." Eur.Respir.J.12. 57-63 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takizawa H: "Drug News Perspect" Cytokines/chemokines and adhesion molecules in local inflammatory responses of the lung., 9 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takizawa H: "International Journal of Molecular Medicine" Airway epithelial cells as regulators of airway inflammation., 12 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-03-31   Modified: 2016-04-21  

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