| Project/Area Number |
10670624
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | HIROSAKI UNIVERSITY |
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Principal Investigator |
HANADA Hiroyuki Hirosaki University, School of Medicine, Instructor, 医学部, 助手 (20250615)
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| Co-Investigator(Kenkyū-buntansha) |
OKUMURA Ken Hirosaki University, School of Medicine, Professor, 医学部, 教授 (20185549)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | basic fibroblast growth factor / coronary collateral circulation / acidic gelatin hydrogel microspheres / regional myocardial blood flow / colored microspheres / colored microsphere |
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| Research Abstract |
Objective: The purpose of this study was to examine whether basic fibroblast growth factor (bFGF)-impregnated acidic gelatin hydrogel microspheres (AGHM) enhance collateral development to the infarct area in dogs with coronary occlusion.
Methods: Studies were conducted in 28 dogs with 2-week occlusion of the proximal left anterior descending coronary artery (LAD). The dogs were divided into 3 groups according to treatment arm (Group A treated with bFGF-impregnated AGHM in the infarct area; Group B with free-form bFGF; Group C with AGHM alone). Coronary angiography (n=15)(Group A, 7 dogs ; Group B, 5 dogs ; Group C, 3 dogs) and regional myocardial blood flow study (n=13)(Group A, 6 dogs ; Group B, 4 dogs ; Group C, 3 dogs) were repeated at a 2-week interval.
Results: Coronary angiography revealed that in Group A, antegrade flow grade in the LAD distal to occlusion which was assessed following Thrombolysis in Myocardial Infarction (TIMI) grade was significantly increased after treatment. In contrast, in Groups B and C, the treatment did not change the flow grade in the LAD. In Group A, the regional myocardial blood flow in the collateral depending area was significantly increased after treatment, and regional myocardial blood flow reserve after adenosine injection was also significantly increased. These measurements remained unchanged despite the treatment in Groups B and C.
Conclusions: Intramyocardial delivery of not free-form bFGF but bFGF-impregnated AGHM improves collateral circulation to the infarct area of dogs with coronary occlusion.
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