| Project/Area Number |
10670638
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
USHIYAMA Yoshihisa Shinshu University, School of Allied Medical Sciensis, Professor, 医療技術短期大学部, 教授 (80020759)
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| Co-Investigator(Kenkyū-buntansha) |
IKOMI Fumitaka Shinshu University, School of Medicine, Assistant Professor, 医学部, 助教授 (50262704)
OHHASHI Toshio Shinshu University, School of Medicine, Professor, 医学部, 教授 (80020832)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | lymphatic endothelial cell / ion channel / patch-clamp / cultured cells / electric physiology / Whole-cell / リンパ管 / 内皮細胞 / パッチクランプ法 / 培養 |
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| Research Abstract |
The patch-clamp technique was employed to measure membrane currents in cultured lymphatic endothelial cells isolated from dog thoracic ducts. Depolarizing.steps positive to -40mV evoked inward currents followed by slow outward currents.
The lymphatic endothelial cells had a mean resting membrane potential of -42.6± 6.4mV( 101 cells ) which was significantly different from the value of -71.5±0.5mV recorded with the glass intracellular microelectrodes in the endothelium of guinea-pig mesenteric lymphatic vessels ( von der Weid, 1997 ).
In whole-cell recording, there was the existence of K+ channel:and Ca2+ channel of the voltage dependency. A voltage-dependent Ca2+ channels followed by rapid inward and slow outward currents here can be called corresponding to a T+ L type channels. On the K+ channel, it seems to be Ca2+-activated K+ channel of' the chemical-stimulated type generally known in the smooth muscle cells, which activates by Ca2+ influx through the voltage-dependent Ca2+ channel and hyper polarize to the membrane potential.
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