| Project/Area Number |
10670646
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KIHARA Yasuki Kyoto University, Grad. School. Med. Assist. Professor, 医学研究科, 助手 (40214853)
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|---|
| Project Period (FY) |
1998 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Cardiac Hypertrophy / Heart Failure / RT-PCR / RT・PCR |
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| Research Abstract |
Using Dahl salt-sensitive rats, we recently developed an animal model in which transition from mechanically compensated concentric LV hypertrophy to its decompensation with LV dysfunction and enlargement was observed in a reproducible manner. In the present study, we explored myocardial mRNA expressions that showed distinct difference between the stages of compensated LVH and LV failure, by means of differential display RT-PCR (DD) methods. We detected 130 mRNA fragments along with the DD comparison and these mRNAs were successfully TA cloned. The subsequent homology search reveled that they consisted of 23 know genes and 14 unknown genes. In the known gene group, mRNAs coding such as atrial natriuretic peptide and alpha-cardiac MHC were found. Interestingly, there were several mRNAs that code factors having been assumed to be independent of heart failure or cardiac remodeling. Thus, we are going to elucidate the pathophysiological roles of these factors coded by these mRNAs on the heart failure.
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